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pp32ΔCT
(Plasmid #67241)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 67241 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pProEX HTb
  • Backbone manufacturer
    life technology
  • Backbone size w/o insert (bp) 4779
  • Total vector size (bp) 5226
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pp32
  • Alt name
    Anp32a
  • Alt name
    acidic nuclear phosphoprotein 32 family member A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    447
  • Mutation
    contains amino acid residues 1-149
  • GenBank ID
    NM_006305
  • Entrez Gene
    ANP32A (a.k.a. C15orf1, HPPCn, I1PP2A, LANP, MAPM, PHAP1, PHAPI, PP32)
  • Tag / Fusion Protein
    • His (N terminal on backbone)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer AGCGGATAACAATTTCACACAGG
  • 3′ sequencing primer ATCTTCTCTCATCCGCCAAAACAG
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pp32ΔCT was a gift from Cynthia Wolberger (Addgene plasmid # 67241 ; http://n2t.net/addgene:67241 ; RRID:Addgene_67241)
  • For your References section:

    The crystal structure of the tumor suppressor protein pp32 (Anp32a): structural insights into Anp32 family of proteins. Huyton T, Wolberger C. Protein Sci. 2007 Jul;16(7):1308-15. Epub 2007 Jun 13. 10.1110/ps.072803507 PubMed 17567741