Purpose(Empty Backbone) EasyClone system-based yeast integrative vector carrying loxP-flanked bleMX marker, integration into S. cerevisiae XI-5 chromosomal location, USER site for cloning, amp resistance
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||67549||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 5795
Vector typeYeast Expression, Cre/Lox ; integrative
Growth in Bacteria
Copy numberHigh Copy
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCfB2196 was a gift from Irina Borodina (Addgene plasmid # 67549 ; http://n2t.net/addgene:67549 ; RRID:Addgene_67549)
For your References section:EasyClone 2.0: expanded toolkit of integrative vectors for stable gene expression in industrial Saccharomyces cerevisiae strains. Stovicek V, Borja GM, Forster J, Borodina I. J Ind Microbiol Biotechnol. 2015 Sep 16. 10.1007/s10295-015-1684-8 PubMed 26376869