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PurposeExpress humanized Cas9 under the control of trpC promoter and terminator from A. nidulans.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 68059 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepRS415
- Total vector size (bp) 11431
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Vector typeYeast Expression, CRISPR ; N. crassa, Fungi
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Selectable markersLEU2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namehumanized Cas9
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SpeciesH. sapiens (human); N. crassa, S.pyogenes, other fungi
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Insert Size (bp)4140
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byAddgene Plasmid #43804
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
p415-GalL-Cas9-CYC1t (Plasmid #43804) from Dr. Church lab was modified to express Cas9 in Fungi.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p415-PtrpC-Cas9-TtrpC-CYC1t was a gift from Christian Hong (Addgene plasmid # 68059 ; http://n2t.net/addgene:68059 ; RRID:Addgene_68059) -
For your References section:
Efficient gene editing in Neurospora crassa with CRISPR technology. Matsu-Ura T, Baek M, Kwon J, Hong C. Fungal Biol Biotechnol. 2015 Jul 15;2:4. doi: 10.1186/s40694-015-0015-1. eCollection 2015. 10.1186/s40694-015-0015-1 PubMed 28955455
Map uploaded by the depositor.
