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p415-PtrpC-Cas9-TtrpC-CYC1t
(Plasmid #68059)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 68059 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS415
  • Total vector size (bp) 11431
  • Vector type
    Yeast Expression, CRISPR ; N. crassa, Fungi
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    humanized Cas9
  • Species
    H. sapiens (human); N. crassa, S.pyogenes, other fungi
  • Insert Size (bp)
    4140

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

p415-GalL-Cas9-CYC1t (Plasmid #43804) from Dr. Church lab was modified to express Cas9 in Fungi.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p415-PtrpC-Cas9-TtrpC-CYC1t was a gift from Christian Hong (Addgene plasmid # 68059 ; http://n2t.net/addgene:68059 ; RRID:Addgene_68059)

  • For your References section:

    Efficient gene editing in Neurospora crassa with CRISPR technology. Matsu-Ura T, Baek M, Kwon J, Hong C. Fungal Biol Biotechnol. 2015 Jul 15;2:4. doi: 10.1186/s40694-015-0015-1. eCollection 2015. 10.1186/s40694-015-0015-1 PubMed 28955455
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