Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Efficient gene editing in Neurospora crassa with CRISPR technology
Matsu-ura Toru, Baek Mokryun, Kwon Jungin and Hong Christian
Fungal Biology and Biotechnology 2015, 2:4

Plasmids from Article

ID Plasmid Purpose  
68059p415-PtrpC-Cas9-TtrpC-CYC1tExpress humanized Cas9 under the control of trpC promoter and terminator from A. nidulans.
68060p426-SNR52p-gRNA.csr-1.Y-SUP4tgRNA for csr-1 locus in N. crassa
89692p426-SNR52p-gRNA.clr-2.Y-SUP4tgRNA for clr-2 locus
89693M249-5'csr-1-Pgsy-1-luciferase-Bar-3'csr-1Luciferase reporter plasmid targeted at csr-1 locus