Efficient gene editing in Neurospora crassa with CRISPR technology.
Matsu-Ura T, Baek M, Kwon J, Hong C
Fungal Biol Biotechnol. 2015 Jul 15;2:4. doi: 10.1186/s40694-015-0015-1. eCollection 2015.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 68059 | p415-PtrpC-Cas9-TtrpC-CYC1t | Express humanized Cas9 under the control of trpC promoter and terminator from A. nidulans. |
| 68060 | p426-SNR52p-gRNA.csr-1.Y-SUP4t | gRNA for csr-1 locus in N. crassa |
| 89692 | p426-SNR52p-gRNA.clr-2.Y-SUP4t | gRNA for clr-2 locus |
| 89693 | M249-5'csr-1-Pgsy-1-luciferase-Bar-3'csr-1 | Luciferase reporter plasmid targeted at csr-1 locus |