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Efficient gene editing in Neurospora crassa with CRISPR technology
Matsu-ura Toru, Baek Mokryun, Kwon Jungin and Hong Christian
Fungal Biology and Biotechnology 2015, 2:4
Article

Plasmids from Article

ID Plasmid Purpose  
68059p415-PtrpC-Cas9-TtrpC-CYC1tExpress humanized Cas9 under the control of trpC promoter and terminator from A. nidulans.
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68060p426-SNR52p-gRNA.csr-1.Y-SUP4tgRNA for csr-1 locus in N. crassa
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89692p426-SNR52p-gRNA.clr-2.Y-SUP4tgRNA for clr-2 locus
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89693M249-5'csr-1-Pgsy-1-luciferase-Bar-3'csr-1Luciferase reporter plasmid targeted at csr-1 locus
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