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Efficient gene editing in Neurospora crassa with CRISPR technology.
Matsu-Ura T, Baek M, Kwon J, Hong C
Fungal Biol Biotechnol. 2015 Jul 15;2:4. doi: 10.1186/s40694-015-0015-1. eCollection 2015. PubMed Article

Plasmids from Article

ID Plasmid Purpose
68059p415-PtrpC-Cas9-TtrpC-CYC1tExpress humanized Cas9 under the control of trpC promoter and terminator from A. nidulans.
68060p426-SNR52p-gRNA.csr-1.Y-SUP4tgRNA for csr-1 locus in N. crassa
89692p426-SNR52p-gRNA.clr-2.Y-SUP4tgRNA for clr-2 locus
89693M249-5'csr-1-Pgsy-1-luciferase-Bar-3'csr-1Luciferase reporter plasmid targeted at csr-1 locus

Antibodies from Article