p426-SNR52p-gRNA.csr-1.Y-SUP4t
(Plasmid #68060)

• Purpose: gRNA for csr-1 locus in N. crassa
• Depositing Lab: Christian Hong
• Publication: Matsu-Ura et al Fungal Biol Biotechnol. 2015 Jul 15;2:4. doi: 10.1186/s40694-015-0015-1. eCollection 2015.

Backbone

• Vector backbone: pRS426
• Total vector size (bp): 6274
• Vector type: Yeast Expression, CRISPR ; N. crassa, Fungi
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: gRNA for csr-1 locus
• Alt name: gRNA
• Alt name: csr-1
• gRNA/shRNA sequence: GAGTGGGAGGGTCCCGTCCT
• Species: N. crassa, S.pyogenes, other fungi
• Promoter: SNR52

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: GGAGCTTCTTTGAAAAGATAATGTATG
• 3′ sequencing primer: GTTACATGCGTACACGCGTC

Resource Information

• A portion of this plasmid was derived from a plasmid made by: p426-SNR52p-gRNA.CAN1.Y-SUP4t (Plasmid #43803)
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The target sequence to csr-1 locus was added instead of CAN1 of p426-SNR52p-gRNA.CAN1.Y-SUP4t (Plasmid #43803) from Dr. Church lab.