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(Plasmid #68060)


Item Catalog # Description Quantity Price (USD)
Plasmid 68060 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Total vector size (bp) 6274
  • Vector type
    Yeast Expression, CRISPR ; N. crassa, Fungi
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    gRNA for csr-1 locus
  • Alt name
  • Alt name
  • gRNA/shRNA sequence
  • Species
    N. crassa, S.pyogenes, other fungi
  • Promoter SNR52

Cloning Information

  • Cloning method Unknown
  • 3′ sequencing primer GTTACATGCGTACACGCGTC
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    p426-SNR52p-gRNA.CAN1.Y-SUP4t (Plasmid #43803)
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The target sequence to csr-1 locus was added instead of CAN1 of p426-SNR52p-gRNA.CAN1.Y-SUP4t (Plasmid #43803) from Dr. Church lab.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p426-SNR52p-gRNA.csr-1.Y-SUP4t was a gift from Christian Hong (Addgene plasmid # 68060 ; ; RRID:Addgene_68060)
  • For your References section:

    Efficient gene editing in Neurospora crassa with CRISPR technology. Matsu-Ura T, Baek M, Kwon J, Hong C. Fungal Biol Biotechnol. 2015 Jul 15;2:4. doi: 10.1186/s40694-015-0015-1. eCollection 2015. 10.1186/s40694-015-0015-1 PubMed 28955455