Purposeexpression of nucleotide free Cdc42 in bacterial cells
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||69356||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4900
- Total vector size (bp) 5500
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)573
Entrez GeneCDC42 (a.k.a. CDC42Hs, G25K, TKS)
- Promoter tac
/ Fusion Protein
- GST (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer pGEX5
- 3′ sequencing primer pGEX3 (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byKeith Burridge Lab, UNC Chapel Hill
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Cdc42 has a mutation, G15A, which makes it nucleotide free when expressed in bacteria, which can be used GEF pulldowns.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGEX-4T1-Cdc42 G15A was a gift from Rafael Garcia-Mata (Addgene plasmid # 69356 ; http://n2t.net/addgene:69356 ; RRID:Addgene_69356)
For your References section:Analysis of activated GAPs and GEFs in cell lysates. Garcia-Mata R, Wennerberg K, Arthur WT, Noren NK, Ellerbroek SM, Burridge K. Methods Enzymol. 2006;406:425-37. 10.1016/S0076-6879(06)06031-9 PubMed 16472675