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pMXs-Puro- MLL3-PRKAG2
(Plasmid #69473)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 69473 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMXs-Puro
  • Backbone manufacturer
    cell biolabs
  • Backbone size w/o insert (bp) 5604
  • Total vector size (bp) 7981
  • Modifications to backbone
    Added a fusion gene and a HA tag
  • Vector type
    Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    200mg/L
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MLL3-PRKAG2
  • Alt name
    MLL3: Lysine N-methyltransferase 2C (KMT2C) also known as myeloid/lymphoid or mixed-lineage leukemia protein 3 (MLL3)
  • Alt name
    PRKAG2: Protein Kinase, AMP-Activated, Gamma 2 Non-Catalytic Subunit
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2377
  • GenBank ID
    NM_170606 NM_001304531
  • Entrez Gene
    PRKAG2 (a.k.a. AAKG, AAKG2, CMH6, H91620p, WPWS)
  • Tag / Fusion Protein
    • HA TAG: (C terminal on insert)

Cloning Information

  • Cloning method TOPO Cloning
  • 5′ sequencing primer TAGAACCTCGCTGGAAAGGA
  • 3′ sequencing primer CGGGACTATGGTTGCTGACT
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMXs-Puro- MLL3-PRKAG2 was a gift from Axel Hillmer (Addgene plasmid # 69473 ; http://n2t.net/addgene:69473 ; RRID:Addgene_69473)
  • For your References section:

    Recurrent Fusion Genes in Gastric Cancer: CLDN18-ARHGAP26 Induces Loss of Epithelial Integrity. Yao F, Kausalya JP, Sia YY, Teo AS, Lee WH, Ong AG, Zhang Z, Tan JH, Li G, Bertrand D, Liu X, Poh HM, Guan P, Zhu F, Pathiraja TN, Ariyaratne PN, Rao J, Woo XY, Cai S, Mulawadi FH, Poh WT, Veeravalli L, Chan CS, Lim SS, Leong ST, Neo SC, Choi PS, Chew EG, Nagarajan N, Jacques PE, So JB, Ruan X, Yeoh KG, Tan P, Sung WK, Hunziker W, Ruan Y, Hillmer AM. Cell Rep. 2015 Jul 14;12(2):272-85. doi: 10.1016/j.celrep.2015.06.020. Epub 2015 Jul 2. 10.1016/j.celrep.2015.06.020 PubMed 26146084