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pMito-eZinCh-2
(Plasmid #70010)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 70010 Standard format: Plasmid sent in bacteria as agar stab 1 $89

Backbone

  • Vector backbone
    pShuttle
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    E. coli nova blue LB culture medium 30 ug/ml kanamycin Culture overnight, 37 C/250 rpm
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mito-eZinch-2
  • Species
    Synthetic
  • Insert Size (bp)
    1800
  • Promoter CMV

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMito-eZinCh-2 was a gift from Maarten Merkx (Addgene plasmid # 70010)

  • For your References section:

    eZinCh-2: A Versatile, Genetically Encoded FRET Sensor for Cytosolic and Intraorganelle Zn(2+) Imaging. Hessels AM, Chabosseau P, Bakker MH, Engelen W, Rutter GA, Taylor KM, Merkx M. ACS Chem Biol. 2015 Sep 18;10(9):2126-34. doi: 10.1021/acschembio.5b00211. Epub 2015 Jul 20. 10.1021/acschembio.5b00211 PubMed 26151333
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