PurposePromoterless AAV plasmid with MCS to insert promoter for expression of EmGFP.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||70119||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression, AAV
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberLow Copy
- Promoter Promoterless
- Cloning method Restriction Enzyme
- 5′ cloning site see map (unknown if destroyed)
- 3′ cloning site see map (unknown if destroyed)
- 5′ sequencing primer M13pUC-rev
- 3′ sequencing primer EBV-rev (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Please see the annotated GenBank file and the additional image file for the specific location of the minipromoter in this plasmid
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pEMS2158 was a gift from Elizabeth Simpson (Addgene plasmid # 70119 ; http://n2t.net/addgene:70119 ; RRID:Addgene_70119)
For your References section:New MiniPromoter Ple345 (NEFL) Drives Strong and Specific Expression in Retinal Ganglion Cells of Mouse and Primate Retina. Simpson E, Korecki AJ, Fornes O, McGill TJ, Cueva-Vargas JL, Agostinone J, Farkas RA, Hickmott JW, Lam SL, Mathelier A, Renner LM, Stoddard J, Zhou M, Di Polo A, Neuringer M, Wasserman WW. Hum Gene Ther. 2018 Jul 31. doi: 10.1089/hum.2018.118. 10.1089/hum.2018.118 PubMed 30062914