pEMS2158
(Plasmid
#70119)
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PurposePromoterless AAV plasmid with MCS to insert promoter for expression of EmGFP.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Plasmid | 70119 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $65 |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneAAV2
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namessAAV-MCS-EmGFP
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Alt nameEmGFP
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SpeciesSynthetic
- Promoter Promoterless
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site see map (unknown if destroyed)
- 3′ cloning site see map (unknown if destroyed)
- 5′ sequencing primer M13pUC-rev
- 3′ sequencing primer EBV-rev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Terms and Licenses
Depositor Comments
Please see the annotated GenBank file and the additional image file for the specific location of the minipromoter in this plasmid
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEMS2158 was a gift from Elizabeth Simpson (Addgene plasmid # 70119 ; http://n2t.net/addgene:70119 ; RRID:Addgene_70119) -
For your References section:
rAAV-compatible MiniPromoters for restricted expression in the brain and eye. de Leeuw CN, Korecki AJ, Berry GE, Hickmott JW, Lam SL, Lengyell TC, Bonaguro RJ, Borretta LJ, Chopra V, Chou AY, D'Souza CA, Kaspieva O, Laprise S, McInerny SC, Portales-Casamar E, Swanson-Newman MI, Wong K, Yang GS, Zhou M, Jones SJ, Holt RA, Asokan A, Goldowitz D, Wasserman WW, Simpson EM. Mol Brain. 2016 May 10;9(1):52. doi: 10.1186/s13041-016-0232-4. 10.1186/s13041-016-0232-4 [pii] PubMed 27164903