pZ005 (AacC2c1 human codon optimized protein)
(Plasmid
#70171)
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PurposeExpresses AacC2c1 human codon optimized protein
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 70171 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneBPV00356 pET21GG2-His(6)-MBP_Flag-Avi
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Backbone manufacturerZhang Lab
- Total vector size (bp) 10239
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameAacC2c1 protein
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Alt nameAacCas12b
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Speciesalicyclobacillus acidoterrestris
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Insert Size (bp)3384
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Tag
/ Fusion Protein
- NLS (C terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pZ005 (AacC2c1 human codon optimized protein) was a gift from Feng Zhang (Addgene plasmid # 70171 ; http://n2t.net/addgene:70171 ; RRID:Addgene_70171) -
For your References section:
Discovery and Functional Characterization of Diverse Class 2 CRISPR-Cas Systems. Shmakov S, Abudayyeh OO, Makarova KS, Wolf YI, Gootenberg JS, Semenova E, Minakhin L, Joung J, Konermann S, Severinov K, Zhang F, Koonin EV. Mol Cell. 2015 Nov 5;60(3):385-97. doi: 10.1016/j.molcel.2015.10.008. Epub 2015 Oct 22. 10.1016/j.molcel.2015.10.008 PubMed 26593719
Map uploaded by the depositor.
