Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

(Plasmid #70699)


Item Catalog # Description Quantity Price (USD)
Plasmid 70699 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    For transformation and plasmid preparation add 0.1 % glucose to the LB medium
  • Copy number
    Low Copy


  • Gene/Insert name
    DASH / Dam1 complex
  • Alt name
    dad1, duo1, spc34, dam1, hsk3, dad4, dad3, dad2, spc19, ask1
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    DAM1 (a.k.a. YGR113W)
  • Entrez Gene
    ASK1 (a.k.a. YKL052C)
  • Entrez Gene
    DUO1 (a.k.a. YGL061C)
  • Entrez Gene
    SPC34 (a.k.a. YKR037C)
  • Entrez Gene
    SPC19 (a.k.a. YDR201W)
  • Entrez Gene
    DAD1 (a.k.a. YDR016C)
  • Entrez Gene
    DAD2 (a.k.a. YKR083C, HSK1)
  • Entrez Gene
    DAD4 (a.k.a. YDR320C-A, HSK2)
  • Entrez Gene
    HSK3 (a.k.a. YKL138C-A)
  • Entrez Gene
    DAD3 (a.k.a. YBR233W-A)
  • Promoter T7
  • Tag / Fusion Protein
    • his-tag on C-terminus of dad1

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Miranda JJ

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PC4_3dad1h was a gift from Stephen Harrison (Addgene plasmid # 70699 ; ; RRID:Addgene_70699)
  • For your References section:

    The yeast DASH complex forms closed rings on microtubules. Miranda JJ, De Wulf P, Sorger PK, Harrison SC. Nat Struct Mol Biol. 2005 Feb;12(2):138-43. Epub 2005 Jan 10. 10.1038/nsmb896 PubMed 15640796