pGEX-KG-mTmod3-WT
(Plasmid #70753)

• Purpose: mouse Tropomodulin3, WT for bacterial expression
• Depositing Lab: Weiping Han
• Publication: Lim et al Nat Commun. 2015 Jan 9;6:5951. doi: 10.1038/ncomms6951.

Backbone

• Vector backbone: pGEX-KG
• Backbone size w/o insert (bp): 5006
• Total vector size (bp): 6053
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: re-transform to BL21 for IPTG-induced protein production
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Tropomodulin3
• Alt name: U-Tmod
• Species: M. musculus (mouse)
• Insert Size (bp): 1059
• Entrez Gene: Tmod3 (a.k.a. U-Tmod, UTMOD)
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhoI (destroyed during cloning)
• 5′ sequencing primer: ggg ctg gca agc cac gtt tgg

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Discrepancies between the full and QC sequences do not have any functional consequence