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(Plasmid #71545)


Item Catalog # Description Quantity Price (USD)
Plasmid 71545 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Chung K-H et al., Polycistronic RNA polymerase II expression vectors for RNA interference based on BIC/miR-155. NAR 2006 vol 24
  • Backbone size w/o insert (bp) 5235
  • Total vector size (bp) 6048
  • Modifications to backbone
    The anti-luciferase cassette was deleted and eGFP was replaced with eGFP that has in the COOH terminus a 20- amino-acid farnesylation signal from c-Ha-Ras. The gene was synthesized for optimal mammalian cell expression
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
    membrane bound eGFP
  • Species
  • Insert Size (bp)
  • GenBank ID
    AEO31555.1 JN255744.1
  • Promoter UbC
  • Tag / Fusion Protein
    • contains 20- amino-acid farnesylation signal from c-Ha-Ras and a 4 aminoacid linker (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer common sequencing primers
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-UbC-eGFP-F was a gift from Pantelis Tsoulfas (Addgene plasmid # 71545 ; ; RRID:Addgene_71545)