Search by Sequence Information

Search by Sequence Terms

Max Results: user-defined upper limit for the number of target sequences returned
Alignment: region of similarity between target and query sequences
E-value: a BLAST statistic representing the significance of an alignment, values close to zero indicate high sequence similarity with low probability of the similarity occurring by chance
Identities: the number of exact nucleotide or amino acid matches over the alignment, expressed as a fraction and a percentage
Query Coverage: the length of the query sequence that matches the target sequence in the alignment
Bit Score: a BLAST statistic measuring the quality of an alignment, higher values indicate a more significant match
Span: the length of the alignment, including gaps

About Search by Sequence

Search by Sequence performs a nucleotide-nucleotide or protein-translated nucleotide BLAST search against Addgene’s plasmid sequence database. BLAST returns plasmids with similarity to the query sequence.
Results are sorted by E-value, a statistic from BLAST that describes the significance of a match. Lower values are considered better matches.
FASTA headers and numbers at the beginning of each line will be removed. The query should only contain DNA characters.

Tips for Success

Enter a distinct sequence that is an important, differentiating feature. For example, the coding region of a gene, instead of the plasmid origin of replication.
Inspect the percent identity, query coverage, and alignment details to determine if a result match is satisfactory. Visit the corresponding plasmid webpage to view additional details about a matching plasmid.
If no results are returned:
- Try a different isoform or region of the desired sequence.
- Choose a different BLAST database. Try the general “All Addgene Plasmids” (default selection), instead of a specific database, such as “Plant Expression Plasmids”
- Try selecting a different BLAST algorithm:
  - megablast: Designed for comparing sequences within the same, or closely related, species. Default selection.
  - blastn: Designed for comparing sequences from different species. May return additional results, if exact species match is not required.
  - blastn-short: Optimized for searching with shorter sequences (<= 30 nucleotides) but can still be effective with slightly larger sequences.
  - tblastn: Designed for comparing protein sequences against a translated nucleotide sequence database. Helpful for finding plasmids with codon-optimized sequences.
  - tblastn-fast: A faster version of tblastn that may return results more quickly, but is less sensitive
- There may not be a match in our database.
You can adjust the Max Results setting on the results page from 25 to 500. If many sequences share the same top E-value, only a truncated set of equally high-scoring matches will be shown. Set the Max Results to 500 to see more matches.
Fill out our Suggest a Plasmid form or e-mail [email protected] to let us know what we're missing.

By using search by sequence, you understand that some sequences are subject to our Terms of Access and the restrictions contained therein.

Search
Search by SequenceNEW

Search

Sequence Type

BLAST Database

BLAST Algorithm

Search plasmids by sequence

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Addgene Website Feedback

Need help? Send us an email at [email protected] Privacy Policy

Skip to main content

This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Menu

Welcome

Sections

Education & Tools

Catalog

Pooled Libraries

By Viral Service

About Our Viral Service

Packaged on Request

Function

Engineered Serotypes

Caltech Systemic

University of Florida Eye Panel

In‐Stock Lentivirus

Pooled CRISPR Libraries

View all lentivirus

γ‐Retrovirus

Fluorescent Proteins

Chemogenetics & Optogenetics

Cloning & Engineering

Bacterial Expression

Empty Backbones

Genetic Code Expansion

Genome Engineering

Tet-Regulated Expression

Research Fields

Allen Institute for Cell Science

View all communities

Available to Industry

Deposit

Depositing with Addgene

Benefits of Depositing Plasmids

Depositor Testimonials

Pre‐Publication Depositing

Start your Deposit

Deposit Overview

Submit Online Deposit

Download Deposit Spreadsheet

Deposit Process Guide

Deposit Help Center Articles

Email [email protected]

Education & Tools

Educational Resources

Molecular Biology Basics

Recipient Instructions

View all Educational Resources

Learn about AAV and antibody materials from user‐contributed reports

Sequence Analyzer

Analyze a DNA sequence to see restriction sites and map

Vector Database

Browse a digital‐only collection of vector backbone information

Developers Portal

Access unparalleled plasmid and sequence data to accelerate your research and fuel groundbreaking discoveries in life sciences and beyond.

Help Center

Ordering & MTAs

How do I place an order? Does Addgene accept orders by fax, phone or email?

What is an MTA/Who is authorized to sign?

How do I get a quote?

What do I need to know about the customs and importation process for my country?

View all Ordering & MTA articles

Technical Support

Can you help me find a plasmid?

There is a problem with the plasmid I received.

My stab did not grow. What do I do?

What strain of bacteria does my stab contain?

How can I be notified when a new material becomes available?

What services does Addgene provide?

View all Technical Support articles

Depositing Plasmids

How do I prepare and deposit my plasmids?

Which plasmids should I deposit?

How can I track requests for my plasmids?

What are Rewards Points?

View all Depositing Plasmids articles

Viral Preps & Antibodies

What is virus associated DNA, and why do I have to order it?

What virus titer will I receive?

Antibody storage

View All Viral Preps & Antibodies articles

Plasmid Handling

Plasmid verification

View all Plasmid Handling articles

Technology Transfer

Resources for your Tech Transfer Office

Access the Help Center

View all Help Center articles

Search Search
Log In
Create Account
Track Order

By Type
Plasmids
Viral Service
Antibodies
Pooled Libraries
Plasmid Kits

By Viral Service
About Our Viral Service
Packaged on Request
In-Stock AAV
- Function
- Biosensors
- Chemogenetics
- Controls
- Optogenetics
- Recombinases
- Engineered Serotypes
- Caltech Systemic
- Retrograde
- University of Florida Eye Panel
- View all AAV
In-Stock Lentivirus

By Plasmid Category

Genome Editing
- CRISPR
- Cre-lox
- TALENs
- Zinc Fingers
Viral Plasmids
Luminescence
- Fluorescent Proteins
- Luciferase
Chemogenetics & Optogenetics
- Chemogenetics
- Optogenetics
Cloning & Engineering

Model Systems
- Microbes
- Plants
- Worm
- Zebrafish
Research Fields
- Cancer
- COVID-19
- Immunology
- Stem Cells
Communities
- Allen Institute for Cell Science
- View all communities
Available to Industry

Depositing with Addgene
Benefits of Depositing Plasmids
Depositor Testimonials
Rewards Points
Pre-Publication Depositing

Start your Deposit
Deposit Overview
Submit Online Deposit
Download Deposit Spreadsheet

Need Help?
Deposit Process Guide
Deposit Help Center Articles
Email [email protected]

Educational Resources

Blog
eBooks
Molecular Biology Basics
Protocols
View all Educational Resources

Recipient Instructions
Science Guides
Videos

Tools

Data Hub
Learn about AAV and antibody materials from user-contributed reports
Sequence Analyzer
Analyze a DNA sequence to see restriction sites and map
Vector Database
Browse a digital-only collection of vector backbone information
Developers Portal
Access unparalleled plasmid and sequence data to accelerate your research and fuel groundbreaking discoveries in life sciences and beyond.

Ordering & MTAs
How do I place an order? Does Addgene accept orders by fax, phone or email?
What is an MTA/Who is authorized to sign?
How do I get a quote?
What do I need to know about the customs and importation process for my country?
View all Ordering & MTA articles

Technical Support
Can you help me find a plasmid?
There is a problem with the plasmid I received.
My stab did not grow. What do I do?
What strain of bacteria does my stab contain?
How can I be notified when a new material becomes available?
What services does Addgene provide?
View all Technical Support articles

Depositing Plasmids
How do I prepare and deposit my plasmids?
Which plasmids should I deposit?
How can I track requests for my plasmids?
What are Rewards Points?
View all Depositing Plasmids articles

Viral Preps & Antibodies
What is virus associated DNA, and why do I have to order it?
What virus titer will I receive?
Viral storage
Antibody storage
View All Viral Preps & Antibodies articles

Plasmid Handling
Bacterial stab
DNA
Pooled library
Kit
Plasmid verification
View all Plasmid Handling articles

Technology Transfer
Resources for your Tech Transfer Office

Access the Help Center
View all Help Center articles

Browse
Jan Kok
Leenhouts et al
71595: pORI28

pORI28 Notes

Addgene Diagnostic Digest

This gel depicts the NEB 1kb ladder and a digestion of the plasmid pORI28 with the following enzyme: EcoRI-HF.

Download Attached File

How was your experience today?

Sign Up for Our Newsletter

Keep up with the latest Addgene news, releases, and more.

Sign Up

Subscribe to Our Blog

Learn about new materials in the repository and helpful community resources.

Subscribe

Contact Addgene

Have questions about your order, deposit, or a material?

Contact Us

Addgene is a nonprofit plasmid repository.

We store and distribute high-quality plasmids from your colleagues.

About Our Repository

Help Center

Deposit Plasmids
- Why Deposit with Us
- Start a Deposit

Browse Our Catalog

General Reagents

Viral Service
- AAV
- Lentivirus

DNA Service
- Cloning Grade DNA

Plasmid Collections

Education

Tools

Stay connected!

Contact
Cookies & Privacy Policy
Accessibility
Site Map
Terms of Use
Status