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• Daniel Zenklusen
• Saroufim et al
• pDZ305 GTL1 GAL1p 24xPP7 V3 <…
• Sequences

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Depositor Full Sequences (1)

Sequences from the depositing laboratory may be predicted or based on Sanger or NGS data. Addgene sequencing results may differ from the depositor-provided sequence.

• 

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  Sequence > pDZ305 GTL1 GAL1p 24xPP7 V3 <HIS> sequence 8242 bps TTAATTAAACCGTTCAACCCTATACAGCGCAAATTTGGTCCTAATACACTTCGGTTTTAATGCGTCAATC CGATTGGCTCCGATAAGCTTTTGCACATTTTTCAAGTCATATGTCACGACGAACGTGATGCCCGCAAAAC GTAAAAAAAAGAAGCAATAAATTGCCCAAAAAAAAAATATAAACTGCTTTTCTTAGTCATTCATAAGTTC TGATGGTCTTCCTTATCTTATTGTATTTTTCGTCCTATTGTTTCATTTCTTACCAAATTAATCAATTCTT ATATCTTACTTGATAACACACCAAACTAATCGTCTCCACATCATAGGAAGATAGGAAATTGCTATCTCAG TCCTATACTACGCAGACCTCGAGTCTGTTTAGCTTGCCTCGTCCCCGCCGGGTCACCCGGCCAGCGACAT GGAGGCCCAGAATACCCTCCTTGACAGTCTTGACGTGCGCAGCTCAGGGGCATGATGTGACTGTCGCCCG TACATTTAGCCCATACATCCCCATGTATAATCATTTGCATCCATACATTTTGATGGCCGCACGGCGCGAA GCAAAAATTACGGCTCCTCGCTGCAGACCTGCGAGCAGGGAAACGCTCCCCTCACAGACGCGTTGAATTG TCCCCACGCCGCGCCCCTGTAGAGAAATATAAAAGGTTAGGATTTGCCACTGAGGTTCTTCTTTCATATA CTTCCTTTTAAAATCTTGCTAGGATACAGTTCTCACATCACATCCGAACATAAACAACCATGGCAGAACC AGCCCAAAAAAAGCAAAAACAAACTGTTCAGGAGCGCAAGGCGTTTATCTCCCGTATCACTAATGAAACT AAAATTCAAATCGCTATTTCGCTGAATGGTGGTTATATTCAAATAAAAGATTCGATTCTTCCTGCAAAGA AGGATGACGATGTAGCTTCCCAAGCTACTCAGTCACAGGTCATCGATATTCACACAGGTGTTGGCTTTTT GGATCATATGATCCATGCGTTGGCAAAACACTCTGGTTGGTCTCTTATTGTTGAATGTATTGGTGACCTG CACATTGACGATCACCATACTACCGAAGATTGCGGTATCGCATTAGGGCAAGCGTTCAAAGAAGCAATGG GTGCTGTCCGTGGTGTAAAAAGATTCGGTACTGGGTTCGCACCATTGGATGAGGCGCTATCACGTGCCGT AGTCGATTTATCTAGTAGACCATTTGCTGTAATCGACCTTGGATTGAAGAGAGAGATGATTGGTGATTTA TCCACTGAAATGATTCCACACTTTTTGGAAAGTTTCGCGGAGGCGGCCAGAATTACTTTGCATGTTGATT GTCTGAGAGGTTTCAACGATCACCACAGAAGTGAGAGTGCGTTCAAGGCTTTGGCTGTTGCCATAAGAGA AGCTATTTCTAGCAATGGCACCAATGACGTTCCCTCAACCAAAGGTGTTTTGATGTGAAGTACTGACAAT AAAAAGATTCTTGTTTTCAAGAACTTGTCATTTGTATAGTTTTTTTATATTGTAGTTGTTCTATTTTAAT CAAATGTTAGCGTGATTTATATTTTTTTTCGCCTCGACATCATCTGCCCAGATGCGAAGTTAAGTGCGCA GAAAGTAATATCATGCGTCAATCGTATGTGAATGCTGGTCGCTATACTGCTGTCGATTCGATACTAACGC CGCCATCCAGAGCTCCAGGTTATCAGCAACAACACAGTCATATCCATTCTCAATTAGCTCTACCACAGTG TGTGAACCAATGTATCCAGCACCACCTGTAACCAAAACAATTTTAGAAGTACTTTCACTTTGTAACTGAG CTGTCATTTATATTGAATTTTCAAAAATTCTTACTTTTTTTTTGGATGGACGCAAAGAAGTTTAATAATC ATATTACATGGCATTACCACCATATACATATCCATATCTAATCTTACTTATATGTTGTGGAAATGTAAAG AGCCCCATTATCTTAGCCTAAAAAAACCTTCTCTTTGGAACTTTCAGTAATACGCTTAACTGCTCATTGC TATATTGAAGTACGGATTAGAAGCCGCCGAGCGGGCGACAGCCCTCCGACGGAAGACTCTCCTCCGTGCG TCCTCGTCTTCACCGGTCGCGTTCCTGAAACGCAGATGTGCCTCGCGCCGCACTGCTCCGAACAATAAAG ATTCTACAATACTAGCTTTTATGGTTATGAAGAGGAAAAATTGGCAGTAACCTGGCCCCACAAACCTTCA AATTAACGAATCAAATTAACAACCATAGGATGATAATGCGATTAGTTTTTTAGCCTTATTTCTGGGGTAA TTAATCAGCGAAGCGATGATTTTTGATCTATTAACAGATATATAAATGGAAAAGCTGCATAACCACTTTA ACTAATACTTTCAACATTTTCAGTTTGTATTACTTCTTATTCAAATGTCATAAAAGTATCAACAAAAAAT TGTTAATATACCTCTATACTTTAACGTCAAGGAGGGATCCTAAGGTACCTAATTGCCTAGAAAGGAGCAG ACGATATGGCGTCGCTCCCTGCAGGTCGACTCTAGAAACCAGCAGAGCATATGGGCTCGCTGGCTGCAGT ATTCCCGGGTTCATTAGATCCTAAGGTACCTAATTGCCTAGAAAGGAGCAGACGATATGGCGTCGCTCCC TGCAGGTCGACTCTAGAAACCAGCAGAGCATATGGGCTCGCTGGCTGCAGTATTCCCGGGTTCATTAGAT CCTAAGGTACCTAATTGCCTAGAAAGGAGCAGACGATATGGCGTCGCTCCCTGCAGGTCGACTCTAGAAA CCAGCAGAGCATATGGGCTCGCTGGCTGCAGTATTCCCGGGTTCATTAGATCCTAAGGTACCTAATTGCC TAGAAAGGAGCAGACGATATGGCGTCGCTCCCTGCAGGTCGACTCTAGAAACCAGCAGAGCATATGGGCT CGCTGGCTGCAGTATTCCCGGGTTCATTAGATCCTAAGGTACCTAATTGCCTAGAAAGGAGCAGACGATA TGGCGTCGCTCCCTGCAGGTCGACTCTAGAAACCAGCAGAGCATATGGGCTCGCTGGCTGCAGTATTCCC GGGTTCATTAGATCCTAAGGTACCTAATTGCCTAGAAAGGAGCAGACGATATGGCGTCGCTCCCTGCAGG TCGACTCTAGAAACCAGCAGAGCATATGGGCTCGCTGGCTGCAGTATTCCCGGGTTCATTAGATCCTAAG GTACCTAATTGCCTAGAAAGGAGCAGACGATATGGCGTCGCTCCCTGCAGGTCGACTCTAGAAACCAGCA GAGCATATGGGCTCGCTGGCTGCAGTATTCCCGGGTTCATTAGATCCTAAGGTACCTAATTGCCTAGAAA GGAGCAGACGATATGGCGTCGCTCCCTGCAGGTCGACTCTAGAAACCAGCAGAGCATATGGGCTCGCTGG CTGCAGTATTCCCGGGTTCATTAGATCCTAAGGTACCTAATTGCCTAGAAAGGAGCAGACGATATGGCGT CGCTCCCTGCAGGTCGACTCTAGAAACCAGCAGAGCATATGGGCTCGCTGGCTGCAGTATTCCCGGGTTC ATTAGATCCTAAGGTACCTAATTGCCTAGAAAGGAGCAGACGATATGGCGTCGCTCCCTGCAGGTCGACT CTAGAAACCAGCAGAGCATATGGGCTCGCTGGCTGCAGTATTCCCGGGTTCATTAGATCCTAAGGTACCT AATTGCCTAGAAAGGAGCAGACGATATGGCGTCGCTCCCTGCAGGTCGACTCTAGAAACCAGCAGAGCAT ATGGGCTCGCTGGCTGCAGTATTCCCGGGTTCATTAGATCCTAAGGTACCTAATTGCCTAGAAAGGAGCA GACGATATGGCGTCGCTCCCTGCAGGTCGACTCTAGAAACCAGCAGAGCATATGGGCTCGCTGGCTGCAG TATTCCCGGGTTCATTAGATCCATGTCCCAGGATAGAATTTTGTTAGATAGATCTATGCCAGTGTTGAAA TCAGACAATTTCGATCCATTGGAAGAAGCTTACGAAGGTGGGACAATTCAAAACTATAACGATGAACACC ATCTTCATAAATCTTGGGCAAATGTGATTCCGGACAAACGAGGACTTTACGACCCTGATTATGAACATGA CGCTTGTGGTGTCGGTTTCGTAGCAAATAAGCATGGTGAACAGTCTCACAAGATTGTTACTGACGCTAGA TATCTTTTAGTGAATATGACACATCGTGGTGCCGTCTCATCTGATGGGAACGGTGACGGTGCCGGTATTC TGCTAGGTATCCCAAGGGCGAATTCGCGGCCGCTAAATTCAATTCGCCCTATAGTGAGTCGTATTACAAT TCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAG CACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCG CAGCCTATACGTACGGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGAT GTACAGAGTGATATTATTGACACGCCGGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGT CAGATAAAGTCTCCCGTGAACTTTACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACCAC CGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATCTCAGCCACCGCGAAAATGAC ATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAAATGTCAGGCATGAGATTATCAAAAAGGATCT TCACCTAGATCCTTTTCACGTAGAAAGCCAGTCCGCAGAAACGGTGCTGACCCCGGATGAATGTCAGCTA CTGGGCTATCTGGACAAGGGAAAACGCAAGCGCAAAGAGAAAGCAGGTAGCTTGCAGTGGGCTTACATGG CGATAGCTAGACTGGGCGGTTTTATGGACAGCAAGCGAACCGGAATTGCCAGCTGGGGCGCCCTCTGGTA AGGTTGGGAAGCCCTGCAAAGTAAACTGGATGGCTTTCTTGCCGCCAAGGATCTGATGGCGCAGGGGATC AAGCTCTGATCAAGAGACAGGATGAGGATCGTTTCGCATGATTGAACAAGATGGATTGCACGCAGGTTCT CCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCG CCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAA TGAACTGCAAGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTC GACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCAT CTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCC GGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGT CTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCA AGGCGAGCATGCCCGACGGCGAGGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGT GGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATA GCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACG GTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAATTATTAA CGCTTACAATTTCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATCAGGTGG CACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCG CTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCT AAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGAT CTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTA CCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCACGCTCACCGGCTCCAGATTTATCAGCAATAAA CCAGCCAGCCGGAAGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATT GTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGG CATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTT ACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGT TGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAG ATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGC TCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAA AACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCG TGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAA AATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATT ATTGAAGCATTTATCAGGGTTATTGTCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGC GTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTG CAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGA AGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCA CTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGT GGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCT GAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCG TGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTC GGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTC GCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAG CAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCC CCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCG AGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTG GCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATT AATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTG GAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTCAGAATTAA CCCTCACTAAAGGGACTAGTCCTGCAGGTTTAAACGAATTCGCCCTTATATA

Addgene-Verified Partial Sequences (2)

Addgene has verified portions of this plasmid. Results are shown below. Primers used for Sanger sequencing are indicated in the sequence header.

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  Sequence > Tef-promoter-F 5'-GGATACAGTTCTCACATCAC-3' ACCAGCCCAAAAAAAGCAAAAACAAACTGTTCAGGAGCGCAAGGCGTTTATCTCCCGTATCACTAATGAA ACTAAAATTCAAATCGCTATTTCGCTGAATGGTGGTTATATTCAAATAAAAGATTCGATTCTTCCTGCAA AGAAGGATGACGATGTAGCTTCCCAAGCTACTCAGTCACAGGTCATCGATATTCACACAGGTGTTGGCTT TTTGGATCATATGATCCATGCGTTGGCAAAACACTCTGGTTGGTCTCTTATTGTTGAATGTATTGGTGAC CTGCACATTGACGATCACCATACTACCGAAGATTGCGGTATCGCATTAGGGCAAGCGTTCAAAGAAGCAA TGGGTGCTGTCCGTGGTGTAAAAAGATTCGGTACTGGGTTCGCACCATTGGATGAGGCGCTATCACGTGC CGTAGTCGATTTATCTAGTAGACCATTTGCTGTAATCGACCTTGGATTGAAGAGAGAGATGATTGGTGAT TTATCCACTGAAATGATTCCACACTTTTTGGAAAGTTTCGCGGAGGCGGCCAGAATTACTTTGCATGTTG ATTGTCTGAGAGGTTTCAACGATCACCACAGAAGTGAGAGTGCGTTCAAGGCTTTGGCTGTTGCCATAAG AGAAGCTATTTCTAGCAATGGCACCAATGACGTTCCCTCAACCAAAGGTGTTTTGATGTGAAGTACTGAC AATAAAAAGATTCTTGTTTTCAAGAACTTGTCATTTGTATAGTTTTTTTATATTGTAGTTGTTCTATTTT AATCAAATGTTAGCGTGATTTATATTTTTTTTCGCCTCGACATCATCTGCCCAGATGCGAAGTTAAGTGC GCAGAAAGTAATATCATGCGTCAATCGTATGTGAATGCTGGTCGCTATACTGCTGTCGATTCGATACTAA CGCCGCCATCCAGAGCTCCAGGTTATCAGCAACAACACAGTCATATCCATTCTCAATTAGCTCTACCACA GTGTG
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  Copy sequence

  Sequence > T7 5'-TAATACGACTCACTATAGGG-3' AATACCGGCACCGTCACCGTTCCCATCAGATGAGACGGCACCACGATGTGTCATATTCACTAAAAGATAT CTAGCGTCAGTAACAATCTTGTGAGACTGTTCACCATGCTTATTTGCTACGAAACCGACACCACAAGCGT CATGTTCATAATCAGGGTCGTAAAGTCCTCGTTTGTCCGGAATCACATTTGCCCAAGATTTATGAAGATG GTGTTCATCGTTATAGTTTTGAATTGTCCCACCTTCGTAAGCTTCTTCCAATGGATCGAAATTGTCTGAT TTCAACACTGGCATAGATCTATCTAACAAAATTCTATCCTGGGACATGGATCTAATGAACCCGGGAATAC TGCAGCCAGCGAGCCCATATGCTCTGCTGGTTTCTAGAGTCGACCTGCAGGGAGCGACGCCATATCGTCT GCTCCTTTCTAGGCAATTAGGTACCTTAGGATCTAATGAACCCGGGAATACTGCAGCCAGCGAGCCCATA TGCTCTGCTGGTTTCTAGAGTCGACCTGCAGGGAGCGACGCCATATCGTCTGCTCCTTTCTAGGCAATTA GGTACCTTAGGATCTAATGAACCCGGGAATACTGCAGCCAGCGAGCCCATATGCTCTGCTGGTTTCTAGA GTCGACCTGCAGGGAGCGACGCCATATCGTCTGCTCCTTTCTAGGCAATTAGGTACCTTAGGATCTAATG AACCCGGGAATACTGCAGCCAGCGAGCCCATATGCTCTGCTGGTTTCTAGAGTCGACCTGCAGGGAGCGA CGCCATATCGTCTGCTCCTTTCTAGGCAATTAGGTACCTTAGGATCTAATGAACCCGGGAATACTGCAGC CAGCGAGCCCATATGCTCTGCTGGTTTCTAGAGTCGACCTGCAGGGAGCGACGCCATATCGTCTGCTCCT TTCTAGGCAATTAGGTACCTTAGGATCTAATGAACCCGGG