PMXS-SLC2A3
(Plasmid #72877)

• Purpose: expresses SLC2A3 in mammalian cells
• Depositing Lab: David Sabatini
• Publication: Birsoy et al Nature. 2014 Apr 3;508(7494):108-12. doi: 10.1038/nature13110. Epub 2014 Mar 16.

Backbone

• Vector backbone: pMXs-IRES-blasticidin
• Backbone size w/o insert (bp): 5639
• Total vector size (bp): 7130
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: SLC2A3
• Alt name: GLUT3
• Species: H. sapiens (human)
• Insert Size (bp): 1491
• GenBank ID: NM_006931.2
• Entrez Gene: SLC2A3 (a.k.a. GLUT3)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH1 (unknown if destroyed)
• 3′ cloning site: EcoR1 (unknown if destroyed)
• 5′ sequencing primer: pLXSN 5' CCCTTGAACCTCCTCGTTCGACC

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The retroviral SLC2A3 vector was generated by cloning into the BamHI and EcoRI sites of the pMXS-ires-blast vector a cDNA insert generated by PCR from a cDNA from Open Biosystems (cat # MHS1010-7429646) using the primers below, followed by standard cloning techniques.SLC2A3 F: GCA TGG ATC CAC CAT GGG CAC ACA GAA GGT CAC
SLC2A3 R: GCA TCA ATT GTT AGA CAT TGG TGG TGG TCT CC