Mouse sgRNA library Brie in lentiGuide-Puro Citations (45)
Originally described in: Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9.Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, Root DE Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437. PubMed Journal
Articles Citing Mouse sgRNA library Brie in lentiGuide-Puro
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| Genome-Wide CRISPR Screen for Essential Cell Growth Mediators in Mutant KRAS Colorectal Cancers. Yau EH, Kummetha IR, Lichinchi G, Tang R, Zhang Y, Rana TM. Cancer Res. 2017 Nov 15;77(22):6330-6339. doi: 10.1158/0008-5472.CAN-17-2043. Epub 2017 Sep 27. PubMed |
| Next-Generation Sequencing of Genome-Wide CRISPR Screens. Yau EH, Rana TM. Methods Mol Biol. 2018;1712:203-216. doi: 10.1007/978-1-4939-7514-3_13. PubMed |
| CRISPR Screens Uncover Genes that Regulate Target Cell Sensitivity to the Morphogen Sonic Hedgehog. Pusapati GV, Kong JH, Patel BB, Krishnan A, Sagner A, Kinnebrew M, Briscoe J, Aravind L, Rohatgi R. Dev Cell. 2018 Jan 8;44(1):113-129.e8. doi: 10.1016/j.devcel.2017.12.003. Epub 2017 Dec 28. PubMed |
| NP220 mediates silencing of unintegrated retroviral DNA. Zhu Y, Wang GZ, Cingoz O, Goff SP. Nature. 2018 Dec;564(7735):278-282. doi: 10.1038/s41586-018-0750-6. Epub 2018 Nov 28. PubMed |
Myc and Dnmt1 impede the pluripotent to totipotent state transition in embryonic stem cells.
Fu X, Wu X, Djekidel MN, Zhang Y.
Nat Cell Biol. 2019 Jul;21(7):835-844. doi: 10.1038/s41556-019-0343-0. Epub 2019 Jun 17.
PubMed
Associated Plasmids |
| Genome-Scale CRISPR Screening for Regulators of Cell Fate Transitions. Carlini V, Gretarsson KH, Hackett JA. Methods Mol Biol. 2021;2214:91-108. doi: 10.1007/978-1-0716-0958-3_7. PubMed |
| Intercellular Mitochondria Transfer to Macrophages Regulates White Adipose Tissue Homeostasis and Is Impaired in Obesity. Brestoff JR, Wilen CB, Moley JR, Li Y, Zou W, Malvin NP, Rowen MN, Saunders BT, Ma H, Mack MR, Hykes BL Jr, Balce DR, Orvedahl A, Williams JW, Rohatgi N, Wang X, McAllaster MR, Handley SA, Kim BS, Doench JG, Zinselmeyer BH, Diamond MS, Virgin HW, Gelman AE, Teitelbaum SL. Cell Metab. 2021 Feb 2;33(2):270-282.e8. doi: 10.1016/j.cmet.2020.11.008. Epub 2020 Dec 4. PubMed |
| The Heme-Regulated Inhibitor Pathway Modulates Susceptibility of Poor Prognosis B-Lineage Acute Leukemia to BH3-Mimetics. Smith KH, Budhraja A, Lynch J, Roberts K, Panetta JC, Connelly JP, Turnis ME, Pruett-Miller SM, Schuetz JD, Mullighan CG, Opferman JT. Mol Cancer Res. 2021 Apr;19(4):636-650. doi: 10.1158/1541-7786.MCR-20-0586. Epub 2020 Dec 7. PubMed |
| Inhibition of ERAD synergizes with FTS to eradicate pancreatic cancer cells. Du R, Sullivan DK, Azizian NG, Liu Y, Li Y. BMC Cancer. 2021 Mar 6;21(1):237. doi: 10.1186/s12885-021-07967-6. PubMed |
| The dystonia gene THAP1 controls DNA double-strand break repair choice. Shinoda K, Zong D, Callen E, Wu W, Dumitrache LC, Belinky F, Chari R, Wong N, Ishikawa M, Stanlie A, Multhaupt-Buell T, Sharma N, Ozelius L, Ehrlich M, McKinnon PJ, Nussenzweig A. Mol Cell. 2021 Jun 17;81(12):2611-2624.e10. doi: 10.1016/j.molcel.2021.03.034. Epub 2021 Apr 14. PubMed |
| An in vivo genome-wide CRISPR screen identifies the RNA-binding protein Staufen2 as a key regulator of myeloid leukemia. Bajaj J, Hamilton M, Shima Y, Chambers K, Spinler K, Van Nostrand EL, Yee BA, Blue SM, Chen M, Rizzeri D, Chuah C, Oehler VG, Broome HE, Sasik R, Scott-Browne J, Rao A, Yeo GW, Reya T. Nat Cancer. 2020 Apr;1(4):410-422. doi: 10.1038/s43018-020-0054-2. Epub 2020 Apr 20. PubMed |
| LKB1 inactivation modulates chromatin accessibility to drive metastatic progression. Pierce SE, Granja JM, Corces MR, Brady JJ, Tsai MK, Pierce AB, Tang R, Chu P, Feldser DM, Chang HY, Bassik MC, Greenleaf WJ, Winslow MM. Nat Cell Biol. 2021 Aug;23(8):915-924. doi: 10.1038/s41556-021-00728-4. Epub 2021 Aug 2. PubMed |
| Keap1 mutation renders lung adenocarcinomas dependent on Slc33a1. Romero R, Sanchez-Rivera FJ, Westcott PMK, Mercer KL, Bhutkar A, Muir A, Gonzalez Robles TJ, Lamboy Rodriguez S, Liao LZ, Ng SR, Li L, Colon CI, Naranjo S, Beytagh MC, Lewis CA, Hsu PP, Bronson RT, Vander Heiden MG, Jacks T. Nat Cancer. 2020 Jun;1(6):589-602. doi: 10.1038/s43018-020-0071-1. Epub 2020 Jun 8. PubMed |
| Single-cell heterogeneity analysis and CRISPR screens in MIN6 cell line reveal transcriptional regulators of insulin. Zhao R, Lu J, Li Q, Xiong F, Zhang Y, Zhu J, Peng G, Yang J. Cell Cycle. 2021 Oct;20(19):2053-2065. doi: 10.1080/15384101.2021.1969204. Epub 2021 Sep 8. PubMed |
| Lrp1 is a host entry factor for Rift Valley fever virus. Ganaie SS, Schwarz MM, McMillen CM, Price DA, Feng AX, Albe JR, Wang W, Miersch S, Orvedahl A, Cole AR, Sentmanat MF, Mishra N, Boyles DA, Koenig ZT, Kujawa MR, Demers MA, Hoehl RM, Moyle AB, Wagner ND, Stubbs SH, Cardarelli L, Teyra J, McElroy A, Gross ML, Whelan SPJ, Doench J, Cui X, Brett TJ, Sidhu SS, Virgin HW, Egawa T, Leung DW, Amarasinghe GK, Hartman AL. Cell. 2021 Sep 30;184(20):5163-5178.e24. doi: 10.1016/j.cell.2021.09.001. Epub 2021 Sep 23. PubMed |
| Mitochondrial respiration contributes to the interferon gamma response in antigen-presenting cells. Kiritsy MC, McCann K, Mott D, Holland SM, Behar SM, Sassetti CM, Olive AJ. Elife. 2021 Nov 2;10. pii: 65109. doi: 10.7554/eLife.65109. PubMed |
| A genetic screen in macrophages identifies new regulators of IFNgamma-inducible MHCII that contribute to T cell activation. Kiritsy MC, Ankley LM, Trombley J, Huizinga GP, Lord AE, Orning P, Elling R, Fitzgerald KA, Olive AJ. Elife. 2021 Nov 8;10. pii: 65110. doi: 10.7554/eLife.65110. PubMed |
| Delivery of CRISPR-Cas9 system for screening and editing RNA binding proteins in cancer. Yan J, Kang DD, Turnbull G, Dong Y. Adv Drug Deliv Rev. 2022 Jan;180:114042. doi: 10.1016/j.addr.2021.114042. Epub 2021 Nov 9. PubMed |
| Fam72a enforces error-prone DNA repair during antibody diversification. Rogier M, Moritz J, Robert I, Lescale C, Heyer V, Abello A, Martin O, Capitani K, Thomas M, Thomas-Claudepierre AS, Laffleur B, Jouan F, Pinaud E, Tarte K, Cogne M, Conticello SG, Soutoglou E, Deriano L, Reina-San-Martin B. Nature. 2021 Dec;600(7888):329-333. doi: 10.1038/s41586-021-04093-y. Epub 2021 Nov 24. PubMed |
| Selective multi-kinase inhibition sensitizes mesenchymal pancreatic cancer to immune checkpoint blockade by remodeling the tumor microenvironment. Falcomata C, Barthel S, Widholz SA, Schneeweis C, Montero JJ, Toska A, Mir J, Kaltenbacher T, Heetmeyer J, Swietlik JJ, Cheng JY, Teodorescu B, Reichert O, Schmitt C, Grabichler K, Coluccio A, Boniolo F, Veltkamp C, Zukowska M, Vargas AA, Paik WH, Jesinghaus M, Steiger K, Maresch R, Ollinger R, Ammon T, Baranov O, Robles MS, Rechenberger J, Kuster B, Meissner F, Reichert M, Flossdorf M, Rad R, Schmidt-Supprian M, Schneider G, Saur D. Nat Cancer. 2022 Mar;3(3):318-336. doi: 10.1038/s43018-021-00326-1. Epub 2022 Jan 31. PubMed |
| Fetal Liver-Derived Alveolar-like Macrophages: A Self-Replicating Ex Vivo Model of Alveolar Macrophages for Functional Genetic Studies. Thomas ST, Wierenga KA, Pestka JJ, Olive AJ. Immunohorizons. 2022 Feb 22;6(2):156-169. doi: 10.4049/immunohorizons.2200011. PubMed |
| ZBP1-dependent inflammatory cell death, PANoptosis, and cytokine storm disrupt IFN therapeutic efficacy during coronavirus infection. Karki R, Lee S, Mall R, Pandian N, Wang Y, Sharma BR, Malireddi RS, Yang D, Trifkovic S, Steele JA, Connelly JP, Vishwanath G, Sasikala M, Reddy DN, Vogel P, Pruett-Miller SM, Webby R, Jonsson CB, Kanneganti TD. Sci Immunol. 2022 Aug 26;7(74):eabo6294. doi: 10.1126/sciimmunol.abo6294. Epub 2022 Aug 26. PubMed |
| mTOR inhibition attenuates chemosensitivity through the induction of chemotherapy resistant persisters. Liu Y, Azizian NG, Sullivan DK, Li Y. Nat Commun. 2022 Nov 17;13(1):7047. doi: 10.1038/s41467-022-34890-6. PubMed |
| A genome-wide CRISPR screen identifies WDFY3 as a regulator of macrophage efferocytosis. Shi J, Wu X, Wang Z, Li F, Meng Y, Moore RM, Cui J, Xue C, Croce KR, Yurdagul A Jr, Doench JG, Li W, Zarbalis KS, Tabas I, Yamamoto A, Zhang H. Nat Commun. 2022 Dec 24;13(1):7929. doi: 10.1038/s41467-022-35604-8. PubMed |
| Systematic identification of factors involved in the silencing of germline genes in mouse embryonic stem cells. Al Adhami H, Vallet J, Schaal C, Schumacher P, Bardet AF, Dumas M, Chicher J, Hammann P, Daujat S, Weber M. Nucleic Acids Res. 2023 Apr 24;51(7):3130-3149. doi: 10.1093/nar/gkad071. PubMed |
| A Genome-Wide Screen in Macrophages Defines Host Genes Regulating the Uptake of Mycobacterium abscessus. Gilliland HN, Beckman OK, Olive AJ. mSphere. 2023 Apr 20;8(2):e0066322. doi: 10.1128/msphere.00663-22. Epub 2023 Feb 16. PubMed |
| Non-canonical functions of SNAIL drive context-specific cancer progression. Paul MC, Schneeweis C, Falcomata C, Shan C, Rossmeisl D, Koutsouli S, Klement C, Zukowska M, Widholz SA, Jesinghaus M, Heuermann KK, Engleitner T, Seidler B, Sleiman K, Steiger K, Tschurtschenthaler M, Walter B, Weidemann SA, Pietsch R, Schnieke A, Schmid RM, Robles MS, Andrieux G, Boerries M, Rad R, Schneider G, Saur D. Nat Commun. 2023 Mar 7;14(1):1201. doi: 10.1038/s41467-023-36505-0. PubMed |
| A multi-omics integrative analysis based on CRISPR screens re-defines the pluripotency regulatory network in ESCs. Ruan Y, Wang J, Yu M, Wang F, Wang J, Xu Y, Liu L, Cheng Y, Yang R, Zhang C, Yang Y, Wang J, Wu W, Huang Y, Tian Y, Chen G, Zhang J, Jian R. Commun Biol. 2023 Apr 14;6(1):410. doi: 10.1038/s42003-023-04700-w. PubMed |
| Parallel Genome-Wide CRISPR Screens to Identify State-Dependent Self-Renewal Regulators of Mouse Embryonic Stem Cells. Wang J, Zhang C, Huang Y, Ruan Y, Hu Y, Wang J, Wang F, Yu M, Xu Y, Liu L, Cheng Y, Yang R, Dong Y, Wang J, Yang Y, Xiong J, Tian Y, Gao Q, Zhang J, Jian R. Stem Cells Dev. 2023 Aug;32(15-16):450-464. doi: 10.1089/scd.2023.0053. Epub 2023 Jun 20. PubMed |
| A genome-wide screen in macrophages identifies PTEN as required for myeloid restriction of Listeria monocytogenes infection. Glover RC, Schwardt NH, Leano SE, Sanchez ME, Thomason MK, Olive AJ, Reniere ML. PLoS Pathog. 2023 May 22;19(5):e1011058. doi: 10.1371/journal.ppat.1011058. eCollection 2023 May. PubMed |
| Therapeutic targeting of metabolic vulnerabilities in cancers with MLL3/4-COMPASS epigenetic regulator mutations. Zhao Z, Cao K, Watanabe J, Philips CN, Zeidner JM, Ishi Y, Wang Q, Gold SR, Junkins K, Bartom ET, Yue F, Chandel NS, Hashizume R, Ben-Sahra I, Shilatifard A. J Clin Invest. 2023 Jul 3;133(13):e169993. doi: 10.1172/JCI169993. PubMed |
| Neuroprotective protein ADNP-dependent histone remodeling complex promotes T helper 2 immune cell differentiation. Ferreira ACF, Szeto ACH, Clark PA, Crisp A, Kozik P, Jolin HE, McKenzie ANJ. Immunity. 2023 Jul 11;56(7):1468-1484.e7. doi: 10.1016/j.immuni.2023.05.010. Epub 2023 Jun 6. PubMed |
| MCK2-mediated MCMV infection of macrophages and virus dissemination to the salivary gland depends on MHC class I molecules. Bosnjak B, Henze E, Lueder Y, Do KTH, Rezalotfi A, Cuvalo B, Ritter C, Schimrock A, Willenzon S, Georgiev H, Fritz L, Galla M, Wagner K, Messerle M, Forster R. Cell Rep. 2023 Jun 7;42(6):112597. doi: 10.1016/j.celrep.2023.112597. PubMed |
| Whole-genome CRISPR screen identifies RAVER1 as a key regulator of RIPK1-mediated inflammatory cell death, PANoptosis. Malireddi RKS, Bynigeri RR, Mall R, Nadendla EK, Connelly JP, Pruett-Miller SM, Kanneganti TD. iScience. 2023 May 22;26(6):106938. doi: 10.1016/j.isci.2023.106938. eCollection 2023 Jun 16. PubMed |
| Genome scale CRISPR screens identify actin capping proteins as key modulators of therapeutic responses to radiation and immunotherapy. Verma N, Renauer PA, Dong C, Xin S, Lin Q, Zhang F, Glazer PM, Chen S. bioRxiv [Preprint]. 2024 Jan 15:2024.01.14.575614. doi: 10.1101/2024.01.14.575614. PubMed |
| NINJ1 mediates inflammatory cell death, PANoptosis, and lethality during infection conditions and heat stress. Han JH, Karki R, Malireddi RKS, Mall R, Sarkar R, Sharma BR, Klein J, Berns H, Pisharath H, Pruett-Miller SM, Bae SJ, Kanneganti TD. Nat Commun. 2024 Feb 26;15(1):1739. doi: 10.1038/s41467-024-45466-x. PubMed |
| Multimodal stimulation screens reveal unique and shared genes limiting T cell fitness. Lin CP, Levy PL, Alflen A, Apriamashvili G, Ligtenberg MA, Vredevoogd DW, Bleijerveld OB, Alkan F, Malka Y, Hoekman L, Markovits E, George A, Traets JJH, Krijgsman O, van Vliet A, Pozniak J, Pulido-Vicuna CA, de Bruijn B, van Hal-van Veen SE, Boshuizen J, van der Helm PW, Diaz-Gomez J, Warda H, Behrens LM, Mardesic P, Dehni B, Visser NL, Marine JC, Markel G, Faller WJ, Altelaar M, Agami R, Besser MJ, Peeper DS. Cancer Cell. 2024 Apr 8;42(4):623-645.e10. doi: 10.1016/j.ccell.2024.02.016. Epub 2024 Mar 14. PubMed |
| Genome-wide screening identifies Trim33 as an essential regulator of dendritic cell differentiation. Tiniakou I, Hsu PF, Lopez-Zepeda LS, Garipler G, Esteva E, Adams NM, Jang G, Soni C, Lau CM, Liu F, Khodadadi-Jamayran A, Rodrick TC, Jones D, Tsirigos A, Ohler U, Bedford MT, Nimer SD, Kaartinen V, Mazzoni EO, Reizis B. Sci Immunol. 2024 Apr 12;9(94):eadi1023. doi: 10.1126/sciimmunol.adi1023. Epub 2024 Apr 12. PubMed |
| In vivo single-cell CRISPR uncovers distinct TNF programmes in tumour evolution. Renz PF, Ghoshdastider U, Baghai Sain S, Valdivia-Francia F, Khandekar A, Ormiston M, Bernasconi M, Dure C, Kretz JA, Lee M, Hyams K, Forny M, Pohly M, Ficht X, Ellis SJ, Moor AE, Sendoel A. Nature. 2024 Aug;632(8024):419-428. doi: 10.1038/s41586-024-07663-y. Epub 2024 Jul 17. PubMed |
| Genome-wide CRISPR-Cas9 knockout screens identify DNMT1 as a druggable dependency in sonic hedgehog medulloblastoma. Tsiami F, Lago C, Pozza N, Piccioni F, Zhao X, Lulsberg F, Root DE, Tiberi L, Kool M, Schittenhelm J, Bandopadhayay P, Segal RA, Tabatabai G, Merk DJ. Acta Neuropathol Commun. 2024 Aug 7;12(1):125. doi: 10.1186/s40478-024-01831-x. PubMed |
| Chromatin Regulator SMARCA4 Is Essential for MHV-Induced Inflammatory Cell Death, PANoptosis. Malireddi RKS, Kanneganti TD. Viruses. 2024 Aug 6;16(8):1261. doi: 10.3390/v16081261. PubMed |
| Mannose receptor (MRC1) mediates uptake of dextran in macrophages via receptor-mediated endocytosis. Wollman J, Wanniarachchi K, Pradhan B, Huang L, Kerkvliet JG, Hoppe AD, Thiex NW. bioRxiv [Preprint]. 2024 Aug 13:2024.08.13.607841. doi: 10.1101/2024.08.13.607841. PubMed |
| Genome-wide screen of Mycobacterium tuberculosis-infected macrophages revealed GID/CTLH complex-mediated modulation of bacterial growth. Simwela NV, Johnston L, Bitar PP, Jaecklein E, Altier C, Sassetti CM, Russell DG. Nat Commun. 2024 Oct 29;15(1):9322. doi: 10.1038/s41467-024-53637-z. PubMed |
| Gliocidin is a nicotinamide-mimetic prodrug that targets glioblastoma. Chen YJ, Iyer SV, Hsieh DC, Li B, Elias HK, Wang T, Li J, Ganbold M, Lien MC, Peng YC, Xie XP, Jayewickreme CD, van den Brink MRM, Brady SF, Lim SK, Parada LF. Nature. 2024 Dec;636(8042):466-473. doi: 10.1038/s41586-024-08224-z. Epub 2024 Nov 20. PubMed |
| Docetaxel response in BRCA1,p53-deficient mammary tumor cells is affected by Huntingtin and BAP1. Gonzalez-Fernandez M, Perry C, Gerhards NM, Francica P, Rottenberg S. Proc Natl Acad Sci U S A. 2024 Dec 24;121(52):e2402849121. doi: 10.1073/pnas.2402849121. Epub 2024 Dec 20. PubMed |
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