PurposeZebrafish transgenic construct
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||74117||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4095
- Total vector size (bp) 5612
Vector typeMammalian Expression ; mRNA generation for microinjection.
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)1521
- Promoter SP6
/ Fusion Protein
- mCerulean 3 (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site Xho1 (not destroyed)
- 5′ sequencing primer Sp6 (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pISceI-myl7:MBIC5-mCer3 was a gift from Michael Tsang (Addgene plasmid # 74117 ; http://n2t.net/addgene:74117 ; RRID:Addgene_74117)
For your References section:A genetically targetable near-infrared photosensitizer. He J, Wang Y, Missinato MA, Onuoha E, Perkins LA, Watkins SC, St Croix CM, Tsang M, Bruchez MP. Nat Methods. 2016 Mar;13(3):263-268. doi: 10.1038/nmeth.3735. Epub 2016 Jan 25. 10.1038/nmeth.3735 PubMed 26808669