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pCR8-Magneto2.0
(Plasmid #74333)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 74333 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Magneto2.0
  • Alt name
    TRPV4 (rat); FTL (human); FTH1(human)
  • Species
    H. sapiens (human), R. norvegicus (rat)
  • Insert Size (bp)
    3642
  • Mutation
    TRPV4: delta 760-871
  • Promoter none
  • Tag / Fusion Protein
    • Flag

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer M13-F
  • 3′ sequencing primer M13R
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCR8-Magneto2.0 was a gift from Ali Guler (Addgene plasmid # 74333)
  • For your References section:

    Genetically targeted magnetic control of the nervous system. Wheeler MA, Smith CJ, Ottolini M, Barker BS, Purohit AM, Grippo RM, Gaykema RP, Spano AJ, Beenhakker MP, Kucenas S, Patel MK, Deppmann CD, Guler AD. Nat Neurosci. 2016 Mar 7. doi: 10.1038/nn.4265. 10.1038/nn.4265 PubMed 26950006