pCAG/hArf4(T31N)-HA
(Plasmid
#79405)
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PurposeExpresses C-terminally HA-tagged Arf4(T31N) in mammalian cells
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Depositing Lab
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Depositing OrganizationKyoto University, Graduate School of Pharmaceutical Sciences
Located in Japan -
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 79405 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 79405-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 79405-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCAG
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameArf4
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SpeciesH. sapiens (human)
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MutationT31N
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Entrez GeneARF4 (a.k.a. ARF2)
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Tag
/ Fusion Protein
- HA (C terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 79405-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 79405-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAG/hArf4(T31N)-HA was a gift from Kazuhisa Nakayama (Addgene plasmid # 79405 ; http://n2t.net/addgene:79405 ; RRID:Addgene_79405) -
For your References section:
ARF1 and ARF4 regulate recycling endosomal morphology and retrograde transport from endosomes to the Golgi apparatus. Nakai W, Kondo Y, Saitoh A, Naito T, Nakayama K, Shin HW. Mol Biol Cell. 2013 Aug;24(16):2570-81. doi: 10.1091/mbc.E13-04-0197. Epub 2013 Jun 19. 10.1091/mbc.E13-04-0197 PubMed 23783033