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pTag-BFP-C-h-Rab4a-c-Myc
(Plasmid #79799)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 79799 Standard format: Plasmid sent in bacteria as agar stab 1 $75 *

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Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Rab4a
  • Species
    H. sapiens (human)
  • Entrez Gene
    RAB4A (a.k.a. HRES-1, HRES-1/RAB4, HRES1, RAB4)
  • Promoter CMV
  • Tags / Fusion Proteins
    • TagBFP (N terminal on backbone)
    • myc (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer BFP-F (CCTTCACCGAGACGCTGTA)
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTag-BFP-C-h-Rab4a-c-Myc was a gift from James Johnson (Addgene plasmid # 79799 ; http://n2t.net/addgene:79799 ; RRID:Addgene_79799)
  • For your References section:

    Inter-domain tagging implicates caveolin-1 in insulin receptor trafficking and Erk signaling bias in pancreatic beta-cells. Boothe T, Lim GE, Cen H, Skovso S, Piske M, Li SN, Nabi IR, Gilon P, Johnson JD. Mol Metab. 2016 Feb 10;5(5):366-78. doi: 10.1016/j.molmet.2016.01.009. eCollection 2016 May. S2212-8778(16)00021-1 [pii] PubMed 27110488