pMSCV-Flag-Mm Ezh2 Y641F
(Plasmid #80077)

• Purpose: Retroviral expression of mouse EZH2 Y641F mutant, hygromycin selection
• Depositing Lab: Phillip Abbosh
• Publication: pMSCV-Flag-Mm Ezh2 Y641F (unpublished)

Backbone

• Vector backbone: MSCVhygro-F-EZH2
• Backbone manufacturer: Addgene distributes
• Total vector size (bp): 9220
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: Flag-EZH2 Y641F
• Alt name: KMT6A
• Species: M. musculus (mouse)
• Insert Size (bp): 2268
• Mutation: Y641F, R342R, N393N
• GenBank ID: NP_031997.2
• Entrez Gene: Ezh2 (a.k.a. Enx-1, Enx1h, KMT6, mKIAA4065)
• Tag / Fusion Protein:
  • 5' FLAG (N terminal on backbone)

Gene/Insert 2

• Gene/Insert name: Hygromycin phosphotransferase
• Alt name: hpt
• Insert Size (bp): 1026

Resource Information

• Supplemental Documents:
  • plasmid map
• A portion of this plasmid was derived from a plasmid made by: Addgene (Kai Ge original depositor)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry