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pCIG-VP16–RAR-alpha
(Plasmid #80434)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 80434 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Retinoic acid receptor alpha
  • Species
    H. sapiens (human)
  • GenBank ID
    NM_000964
  • Entrez Gene
    RARA (a.k.a. NR1B1, RAR)
  • Promoter beta-actin promoter
  • Tags / Fusion Proteins
    • VP16 (C terminal on insert)
    • EGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (unknown if destroyed)
  • 3′ cloning site EcoRV (unknown if destroyed)
  • 5′ sequencing primer pCAG-F
  • 3′ sequencing primer pCDH-rev
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCIG-VP16–RAR-alpha was a gift from Aixa Morales (Addgene plasmid # 80434 ; http://n2t.net/addgene:80434 ; RRID:Addgene_80434)
  • For your References section:

    FGF and retinoic acid activity gradients control the timing of neural crest cell emigration in the trunk. Martinez-Morales PL, Diez del Corral R, Olivera-Martinez I, Quiroga AC, Das RM, Barbas JA, Storey KG, Morales AV. J Cell Biol. 2011 Aug 8;194(3):489-503. doi: 10.1083/jcb.201011077. Epub 2011 Aug 1. 10.1083/jcb.201011077 PubMed 21807879