PurposeExpression of constitutively active human RAR-alpha in chick embryos
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||80434||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6000
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameRetinoic acid receptor alpha
SpeciesH. sapiens (human)
Entrez GeneRARA (a.k.a. NR1B1, RAR)
- Promoter beta-actin promoter
/ Fusion Proteins
- VP16 (C terminal on insert)
- EGFP (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRV (unknown if destroyed)
- 3′ cloning site EcoRV (unknown if destroyed)
- 5′ sequencing primer pCAG-F
- 3′ sequencing primer pCDH-rev (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made bypW1Act-VP16.hRARα insert was originally provided by Michael Underhill. We subsequently cloned this insert into PCIG.
Terms and Licenses
- Not Available to Industry
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCIG-VP16–RAR-alpha was a gift from Aixa Morales (Addgene plasmid # 80434 ; http://n2t.net/addgene:80434 ; RRID:Addgene_80434)
For your References section:FGF and retinoic acid activity gradients control the timing of neural crest cell emigration in the trunk. Martinez-Morales PL, Diez del Corral R, Olivera-Martinez I, Quiroga AC, Das RM, Barbas JA, Storey KG, Morales AV. J Cell Biol. 2011 Aug 8;194(3):489-503. doi: 10.1083/jcb.201011077. Epub 2011 Aug 1. 10.1083/jcb.201011077 PubMed 21807879