Purposecreate protein aggregates in C. elegans
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||80626||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonemodified worm expression vector with Pvha6 promoter
- Backbone size w/o insert (bp) 4607
- Total vector size (bp) 5697
Modifications to backbonesame insert as Plasmid #78289 (AgDD), but with with Pvha6 promoter for worm expression.
Vector typeWorm Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)1087
GenBank IDNM_000801 AB971579
Entrez GeneFKBP1A (a.k.a. FKBP-12, FKBP-1A, FKBP1, FKBP12, PKC12, PKCI2, PPIASE)
/ Fusion Protein
- MLALKLAGLDI (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site NcoI (not destroyed)
- 5′ sequencing primer unknown
- 3′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
The depositor-provided reference sequence shows 3 additional nucleotide mismatches compared to listed NM_000801 and also F36V compared to the respective protein (NP_000792.1). Addgene Sanger sequencing confirmed these differences.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Pvha6-AgDD was a gift from Thomas Wandless (Addgene plasmid # 80626 ; http://n2t.net/addgene:80626 ; RRID:Addgene_80626)
For your References section:A method to rapidly create protein aggregates in living cells. Miyazaki Y, Mizumoto K, Dey G, Kudo T, Perrino J, Chen LC, Meyer T, Wandless TJ. Nat Commun. 2016 May 27;7:11689. doi: 10.1038/ncomms11689. 10.1038/ncomms11689 PubMed 27229621