M13KO7-ΔM13-ΔgeneIII-ΔgeneVI
(Plasmid #80840)

• Purpose: Modified helper phage
• Depositing Lab: Mark Isalan
• Publication: Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10.1038/ncomms13858.

Backbone

• Vector backbone: M13KO7
• Total vector size (bp): 6836
• Modifications to backbone: Deletion of M13 packaging signal Deletion of M13 geneIII and gene VI
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha ; TOP 10
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: M13 gene II
• Species: M13 bacteriophage
• Insert Size (bp): 1235

Gene/Insert 2

• Gene/Insert name: M13 gene X
• Species: M13 bacteriophage
• Insert Size (bp): 336

Gene/Insert 3

• Gene/Insert name: M13 gene V
• Species: M13 bacteriophage
• Insert Size (bp): 264

Gene/Insert 4

• Gene/Insert name: M13 gene VII
• Species: M13 bacteriophage
• Insert Size (bp): 102

Gene/Insert 5

• Gene/Insert name: M13 gene IX
• Species: M13 bacteriophage
• Insert Size (bp): 99

Gene/Insert 6

• Gene/Insert name: M13 gene VIII
• Species: M13 bacteriophage
• Insert Size (bp): 222

Gene/Insert 7

• Gene/Insert name: M13 gene I
• Species: M13 bacteriophage
• Insert Size (bp): 1047

Gene/Insert 8

• Gene/Insert name: M13 gene XI
• Species: M13 bacteriophage
• Insert Size (bp): 327

Gene/Insert 9

• Gene/Insert name: M13 gene IV
• Species: M13 bacteriophage
• Insert Size (bp): 1281

Resource Information

• Supplemental Documents:
  • Annotated GenBank File 80840
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry