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(Plasmid #83114)


Item Catalog # Description Quantity Price (USD)
Plasmid 83114 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Vector type
    Toxoplasma gondii
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer TACTTCCAATCCAATTTAGC
  • 3′ sequencing primer TCCTCCACTTCCAATTTTAGC
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYFP.LIC.DHFR was a gift from Vernon Carruthers (Addgene plasmid # 83114 ; ; RRID:Addgene_83114)
  • For your References section:

    Tagging of endogenous genes in a Toxoplasma gondii strain lacking Ku80. Huynh MH, Carruthers VB. Eukaryot Cell. 2009 Apr;8(4):530-9. doi: 10.1128/EC.00358-08. Epub 2009 Feb 13. 10.1128/EC.00358-08 PubMed 19218426