PurposeUsed to evaluate the expression output of CUP1 promoter with yEGFP used as the reporter gene.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||83555||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeYeast Expression
Selectable markersURA3 ; kluyveromyces lactis in S. cerevisiae
Growth in Bacteria
Growth instructionsalpha-Select from BIOLINE (Genotype: F- deoR endA1 recA1 relA1 gyrA96 hsdR17(rk-, mk+) supE44 thi-1 phoA Δ(lacZYA-argF)U169 Φ80lacZΔM15 λ-)
Copy numberHigh Copy
Gene/Insert nameCUP1 promoter-yEGFP
SpeciesS. cerevisiae (budding yeast)
- Promoter CUP1
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer unknown
- 3′ sequencing primer yGFP-R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Please note that the promoters were cloned from strain CEN.PK113-7D and the assembled full sequence are based on S288C promoters. Discrepancies in these promoters are the SNPs in CEN.PK113-7D.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pILGFPI7 was a gift from Claudia Vickers (Addgene plasmid # 83555 ; http://n2t.net/addgene:83555 ; RRID:Addgene_83555)
For your References section:Controlling heterologous gene expression in yeast cell factories on different carbon substrates and across the diauxic shift: a comparison of yeast promoter activities. Peng B, Williams TC, Henry M, Nielsen LK, Vickers CE. Microb Cell Fact. 2015 Jun 26;14:91. doi: 10.1186/s12934-015-0278-5. 10.1186/s12934-015-0278-5 PubMed 26112740