pB_tetO7_sfGFP
(Plasmid
#196616)
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PurposePlasmid for integrating tetO7-controlled sfGFP into the HO region in yeast
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 196616 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneHO-poly-KanMX4-HO (ATCC 87804)
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Backbone manufacturerATCC
- Backbone size w/o insert (bp) 4573
- Total vector size (bp) 9527
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Vector typeYeast Expression
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Selectable markersKanMX (select with Geneticin/G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Growth instructionsNEB5a strain
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSuperfolder GFP
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)4954
- Promoter tetO7 promoter
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer AATTATCCTGGGCACGAG
- 3′ sequencing primer ACTGTAAGATTCCGCCAC (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Supplementary Table 2 of the paper provides details on the cloning of the plasmid.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pB_tetO7_sfGFP was a gift from Matthias Heinemann (Addgene plasmid # 196616 ; http://n2t.net/addgene:196616 ; RRID:Addgene_196616) -
For your References section:
Temporal segregation of biosynthetic processes is responsible for metabolic oscillations during the budding yeast cell cycle. Takhaveev V, Ozsezen S, Smith EN, Zylstra A, Chaillet ML, Chen H, Papagiannakis A, Milias-Argeitis A, Heinemann M. Nat Metab. 2023 Feb;5(2):294-313. doi: 10.1038/s42255-023-00741-x. Epub 2023 Feb 27. 10.1038/s42255-023-00741-x PubMed 36849832
