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pLenti Dest PuroR R1-R2
(Plasmid #84575)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 84575 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMuLE Lenti Dest Neo
  • Backbone size (bp) 10359
  • Modifications to backbone
    Subcloned in PuroR using SbfI and MluI
  • Vector type
    Mammalian Expression, Lentiviral ; Gateway
  • Promoter PGK
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Dual selection with chloramphenicol and ampicillin required to prevent recombination
  • Copy number
    Low Copy

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Ian Frew, Addgene #62178

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti Dest PuroR R1-R2 was a gift from Ghassan Mouneimne (Addgene plasmid # 84575 ; http://n2t.net/addgene:84575 ; RRID:Addgene_84575)
  • For your References section:

    The actin cytoskeletal architecture of estrogen receptor positive breast cancer cells suppresses invasion. Padilla-Rodriguez M, Parker SS, Adams DG, Westerling T, Puleo JI, Watson AW, Hill SM, Noon M, Gaudin R, Aaron J, Tong D, Roe DJ, Knudsen B, Mouneimne G. Nat Commun. 2018 Jul 30;9(1):2980. doi: 10.1038/s41467-018-05367-2. 10.1038/s41467-018-05367-2 [pii] PubMed 30061623