PurposeTG2 biosensor that allows for quantitative assessment of TG2 conformational changes in live cells using FRET
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||85471||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Modifications to backboneeYFP insert cloned into BamHI and XbaI sites at the 3' end of the multiple cloning site (MCS).
Vector typeMammalian Expression
Growth in Bacteria
Alt nametransglutaminase 2
SpeciesH. sapiens (human)
Entrez GeneTGM2 (a.k.a. TG(C), TGC)
- Promoter CMV
/ Fusion Proteins
- mCerulean (N terminal on backbone)
- EYFP (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (unknown if destroyed)
- 3′ cloning site Acc65I (unknown if destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer EGFP-N (Common Sequencing Primers)
Terms and Licenses
The hTG2 insert contains V224G and A141T mutations. The depositor states that these mutations do not affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:mCer-TG2-YFP was a gift from Jeffrey Keillor & Ray Truant (Addgene plasmid # 85471 ; http://n2t.net/addgene:85471 ; RRID:Addgene_85471)
For your References section:Using FLIM-FRET to measure conformational changes of transglutaminase type 2 in live cells. Caron NS, Munsie LN, Keillor JW, Truant R. PLoS One. 2012;7(8):e44159. doi: 10.1371/journal.pone.0044159. Epub 2012 Aug 31. 10.1371/journal.pone.0044159 PubMed 22952912