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mCer-TG2-YFP
(Plasmid #85471)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 85471 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    mCerulean-C1
  • Modifications to backbone
    eYFP insert cloned into BamHI and XbaI sites at the 3' end of the multiple cloning site (MCS).
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    hTG2
  • Alt name
    transglutaminase 2
  • Species
    H. sapiens (human)
  • Entrez Gene
    TGM2 (a.k.a. TG(C), TGC)
  • Promoter CMV
  • Tags / Fusion Proteins
    • mCerulean (N terminal on backbone)
    • EYFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (unknown if destroyed)
  • 3′ cloning site Acc65I (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer EGFP-N
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

The hTG2 insert contains V224G and A141T mutations. The depositor states that these mutations do not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mCer-TG2-YFP was a gift from Jeffrey Keillor & Ray Truant (Addgene plasmid # 85471 ; http://n2t.net/addgene:85471 ; RRID:Addgene_85471)
  • For your References section:

    Using FLIM-FRET to measure conformational changes of transglutaminase type 2 in live cells. Caron NS, Munsie LN, Keillor JW, Truant R. PLoS One. 2012;7(8):e44159. doi: 10.1371/journal.pone.0044159. Epub 2012 Aug 31. 10.1371/journal.pone.0044159 PubMed 22952912