pET28a SUMO Nup53xl 162-267
Purpose(Empty Backbone) bacterial expression of his6 and SUMO tagged RRM motif of Xenopus laevis Nup53
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||86002||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 5300
Modifications to backboneremoval of the N-Terminal HIS6 tag, addition of teh MISTIC sequence upstream of the MCS
Vector typeBacterial Expression
/ Fusion Protein
- SUMO (N terminal on insert)
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberLow Copy
- Cloning method Unknown
A portion of this plasmid was derived from a plasmid made bywe received the original MISTIC seuqnce from Senyon Choe, Salk Institute
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pET28a SUMO Nup53xl 162-267 was a gift from Wolfram Antonin (Addgene plasmid # 86002 ; http://n2t.net/addgene:86002 ; RRID:Addgene_86002)