pStI6
(Plasmid #8616)

• Depositing Lab: Robert Hartley
• Publication: Krajcikova et al FEBS Lett 2004 Jan 16;557(1-3):164-8.

Backbone

• Vector backbone: pET-28a
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5252
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Ribonuclease inhibitor StI
• Species: Saccharopolyspora erythraea, Strain NRRL 2338
• Insert Size (bp): 365
• Mutation: S2G point mutation; 19 silent mutations improve production in E. coli.

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Production plasmid for ribonuclease inhibitor and barstar
homolog StI from Saccharopolyspora erythraea. Structural gene for inhibitor StI from Saccharopolyspora
erythraea strain NRRL 2338. N-terminal serine of wild type
changed to glycine and 19 silent mutations improve production
in E. coli.