pcDNA3.1-LGC-53
(Plasmid #86399)

• Purpose: Mammalian expression of codon-optimized dopamine receptor LGC-53
• Depositing Lab: Steven Mennerick
• Publication: Kress et al J Neurosci. 2014 Aug 27;34(35):11792-802. doi: 10.1523/JNEUROSCI.2355-14.2014.

Backbone

• Vector backbone: pcDNA3.1(+)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5357
• Total vector size (bp): 6884
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: LGC-53
• Alt name: Ligand-gated chloride channel
• Species: C. elegans (nematode), Synthetic
• Insert Size (bp): 1528
• Mutation: Codon optimized for mammalian expression; Signal sequence optimized to that of rat Gabra1. Amino acid changes R398A, R399A, R400A, R439A, R440A, R441A.
• Entrez Gene: lgc-53 (a.k.a. CELE_T21F2.1)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Supplemental Documents:
  • 86399.gb
• A portion of this plasmid was derived from a plasmid made by: Insert was created by Epoch Life Science, Inc (1306 FM-1092, Suite 407, Missouri City, TX 77459

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry