pLVTHM-shRnd3
(Plasmid #86437)

• Purpose: 2nd generation doxycycline-inducible lentiviral vector expressing Rnd3-targeting shRNA and GFP
• Depositing Lab: Violaine Moreau
• Publication: Grise et al Hepatology. 2012 Jun;55(6):1766-75. doi: 10.1002/hep.25568.

Backbone

• Vector backbone: pLVTHM
• Backbone manufacturer: Didier Trono Lab (Addgene #12247)
• Vector type: Mammalian Expression, Lentiviral, RNAi

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: shRNA targeting RND3
• gRNA/shRNA sequence: TAGTAGAGCTCTCCAATCACA
• Species: H. sapiens (human)
• Entrez Gene: RND3 (a.k.a. ARHE, Rho8, RhoE, memB)
• Promoter: H1

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (unknown if destroyed)
• 3′ cloning site: MluI (unknown if destroyed)
• 5′ sequencing primer: H1 (5' tcgctatgtgttctgggaaa 3')
• 3′ sequencing primer: SP6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry