piRFP670-N1-GalT
(Plasmid
#87325)
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PurposeTo express GalT-iRFP670 in mammalian cells, GalT encodes the 1-81 amino acids from β1,4-galactosyltransferase
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 87325 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepiRFP670-N1
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Backbone manufacturerVladislav Verkhusha Lab
- Backbone size w/o insert (bp) 4948
- Total vector size (bp) 5191
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name1-81 amino acids from β1,4-galactosyltransferase
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SpeciesH. sapiens (human)
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Insert Size (bp)243
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GenBank IDNM_001497.3
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Entrez GeneB4GALT1 (a.k.a. B4GAL-T1, CDG2D, GGTB2, GT1, GTB, beta4Gal-T1)
- Promoter CMV
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Tag
/ Fusion Protein
- iRFP670 (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
piRFP670-N1-GalT was a gift from Lei Lu (Addgene plasmid # 87325 ; http://n2t.net/addgene:87325 ; RRID:Addgene_87325) -
For your References section:
A novel imaging method for quantitative Golgi localization reveals differential intra-Golgi trafficking of secretory cargos. Tie HC, Mahajan D, Chen B, Cheng L, VanDongen AM, Lu L. Mol Biol Cell. 2016 Jan 13. pii: mbc.E15-09-0664. 10.1091/mbc.E15-09-0664 PubMed 26764092