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Addgene

piRFP670-N1-GalT
(Plasmid #87325)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 87325 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    piRFP670-N1
  • Backbone manufacturer
    Vladislav Verkhusha Lab
  • Backbone size w/o insert (bp) 4948
  • Total vector size (bp) 5191
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    1-81 amino acids from β1,4-galactosyltransferase
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    243
  • GenBank ID
    NM_001497.3
  • Entrez Gene
    B4GALT1 (a.k.a. B4GAL-T1, CDG2D, GGTB2, GT1, GTB, beta4Gal-T1)
  • Promoter CMV
  • Tag / Fusion Protein
    • iRFP670 (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    piRFP670-N1-GalT was a gift from Lei Lu (Addgene plasmid # 87325 ; http://n2t.net/addgene:87325 ; RRID:Addgene_87325)
  • For your References section:

    A novel imaging method for quantitative Golgi localization reveals differential intra-Golgi trafficking of secretory cargos. Tie HC, Mahajan D, Chen B, Cheng L, VanDongen AM, Lu L. Mol Biol Cell. 2016 Jan 13. pii: mbc.E15-09-0664. 10.1091/mbc.E15-09-0664 PubMed 26764092