|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||8734||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4738
Vector typeYeast Expression
Selectable markersS. pombe HIS5
Growth in Bacteria
Gene/Insert nameyECitrine A206R
Alt nameCodon-optimized GFP
MutationyEGFP with S65G, V68L, Q69M, S72A, T203Y, A206R
/ Fusion Protein
- Codon optimized linker (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ sequencing primer N/A (Common Sequencing Primers)
Terms and Licenses
Full name of this plasmid is pFA6a-link-yECitrineA206R-SpHIS5. yECitrine A206R is created by mutagenesis from a codon-optimized green
fluorescent protein - yEGFP1 (Cormack et al. 1997). This yEGFP
variant is cloned into pDH5, replacing YFP. An codon-optimized linker
was added into the yEGFP variant to improve expression level. The
selectable marker, Sz. pombe HIS5 (SpHIS5), complements S. cerevisiae
HIS3. The yEmCFP and yEmCitrine proteins contain the A206R mutation to block the weak dimerization of the parent GFP (see Zacharias et al., Science 2002 296: 913-916).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pKT0211 was a gift from Kurt Thorn (Addgene plasmid # 8734)
For your References section:Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae. Sheff MA, Thorn KS. Yeast 2004 Jun;21(8):661-70. 10.1002/yea.1130 PubMed 15197731
Generated by Addgene from full sequence supplied by depositor.