FRB-CFP-ras
(Plasmid
#87451)
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Purposerapamycin binding motif of mTOR (FRB) anchored to the plasma membrane
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Depositing Lab
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Depositing OrganizationTsinghua University
Located in China -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 87451 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 87451-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 87451-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepcDNA3
- Backbone size w/o insert (bp) 5388
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFRB
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SpeciesH. sapiens (human)
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Insert Size (bp)282
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MutationFKBP and rapamycin binding domain only
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Entrez GeneMTOR (a.k.a. FRAP, FRAP1, FRAP2, RAFT1, RAPT1, SKS)
- Promoter CMV
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Tags
/ Fusion Proteins
- ras fragment (C terminal on insert)
- CFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Bam HI (not destroyed)
- 3′ cloning site Xho I (not destroyed)
- 5′ sequencing primer CMV-F
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
ras fragment (60 bp)
DNA (Catalog # 87451-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 87451-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FRB-CFP-ras was a gift from Xiaodong Liu (Addgene plasmid # 87451 ; http://n2t.net/addgene:87451 ; RRID:Addgene_87451) -
For your References section:
Cooperative and acute inhibitionby multiple C-terminal motifs of L-type Ca2+ channels. Liu N, Yang Y, Ge L, Liu M, Colecraft HM, Liu X. Elife. 2017 Jan 6;6. pii: e21989. doi: 10.7554/eLife.21989. 10.7554/eLife.21989 PubMed 28059704
Map uploaded by the depositor.
