3185 pBS Bax cDNA
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||8754||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepBluescript II KS(+)
- Backbone size w/o insert (bp) 3000
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesM. musculus (mouse)
Insert Size (bp)800
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
for in situ probes: antisense - cut with PstI and use T7 polymerase. sense - cut with SalI and use T3 polymerase.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:3185 pBS Bax cDNA was a gift from Stanley Korsmeyer (Addgene plasmid # 8754 ; http://n2t.net/addgene:8754 ; RRID:Addgene_8754)
For your References section:Bax-deficient mice with lymphoid hyperplasia and male germ cell death. Knudson CM, Tung KS, Tourtellotte WG, Brown GA, Korsmeyer SJ. Science 1995 Oct 6;270(5233):96-9. 10.1126/science.270.5233.96 PubMed 7569956
Map uploaded by the depositor.