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p-EGFP-C1-Flag-mFmr1(A)
(Plasmid #87913)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 87913 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    BD Biosciences
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 6566
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Fmr1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1866
  • Mutation
    S499A
  • Entrez Gene
    Fmr1 (a.k.a. FM, FMRP, Fmr-, Fmr-1)
  • Tags / Fusion Proteins
    • Flag (N terminal on insert)
    • eGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SacII (not destroyed)
  • 5′ sequencing primer EGFP-C
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p-EGFP-C1-Flag-mFmr1(A) was a gift from Stephanie Ceman (Addgene plasmid # 87913 ; http://n2t.net/addgene:87913 ; RRID:Addgene_87913)
  • For your References section:

    Identification and characterization of the methyl arginines in the fragile X mental retardation protein Fmrp. Stetler A, Winograd C, Sayegh J, Cheever A, Patton E, Zhang X, Clarke S, Ceman S. Hum Mol Genet. 2006 Jan 1;15(1):87-96. Epub 2005 Nov 30. 10.1093/hmg/ddi429 PubMed 16319129