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Addgene

pYTP MYRF sg5
(Plasmid #88861)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 88861 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAC154-dual-dCas9VP160-sgExpression
  • Backbone manufacturer
    Rudolf Jaenisch (Addgene plasmid # 48240)
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dCas9VP160 and MYRF sg5 gRNA
  • gRNA/shRNA sequence
    GCCCTGTGCCGGTGGTGGGC
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    20
  • Entrez Gene
    MYRF (a.k.a. C11orf9, MRF, Ndt80, pqn-47)
  • Promoter U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BbsI (destroyed during cloning)
  • 3′ cloning site BbsI (destroyed during cloning)
  • 5′ sequencing primer LKO5
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYTP MYRF sg5 was a gift from Mahmoud Pouladi (Addgene plasmid # 88861 ; http://n2t.net/addgene:88861 ; RRID:Addgene_88861)
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