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pMX-IY LC3B
(Plasmid #89291)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 89291 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LC3B
  • Alt name
    MAP1LC3B
  • Alt name
    ATG8F
  • Species
    H. sapiens (human)
  • Entrez Gene
    MAP1LC3B (a.k.a. ATG8F, LC3B, MAP1A/1BLC3, MAP1LC3B-a)
  • Tag / Fusion Protein
    • IRES-YFP (C terminal on backbone)

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMX-IY LC3B was a gift from Michael Lazarou (Addgene plasmid # 89291 ; http://n2t.net/addgene:89291 ; RRID:Addgene_89291)
  • For your References section:

    Atg8 family LC3/GABARAP proteins are crucial for autophagosome-lysosome fusion but not autophagosome formation during PINK1/Parkin mitophagy and starvation. Nguyen TN, Padman BS, Usher J, Oorschot V, Ramm G, Lazarou M. J Cell Biol. 2016 Dec 19;215(6):857-874. Epub 2016 Nov 18. 10.1083/jcb.201607039 PubMed 27864321