Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pTRE/SP M87 WT
(Plasmid #89328)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 89328 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pTRE-Tight
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 2600
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    spastin
  • Alt name
    SPAST
  • Alt name
    NM 014946
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1668
  • Mutation
    1-460 bp deleted
  • Entrez Gene
    SPAST (a.k.a. ADPSP, FSP2, SPG4)
  • Promoter Tet-responsive P tight

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SmaI (destroyed during cloning)
  • 3′ cloning site BamH I (destroyed during cloning)
  • 5′ sequencing primer LNCX
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

The endogenous M87 Kozak sequence was replaced with an optimal Kozak sequence. To detect spastin use an anti-spastin antibody.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTRE/SP M87 WT was a gift from Peter Baas (Addgene plasmid # 89328 ; http://n2t.net/addgene:89328 ; RRID:Addgene_89328)
  • For your References section:

    Pathogenic mutation of spastin has gain-of-function effects on microtubule dynamics. Solowska JM, D'Rozario M, Jean DC, Davidson MW, Marenda DR, Baas PW. J Neurosci. 2014 Jan 29;34(5):1856-67. doi: 10.1523/JNEUROSCI.3309-13.2014. 10.1523/JNEUROSCI.3309-13.2014 PubMed 24478365