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pIRES RED/SP M1C448Y
(Plasmid #89323)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 89323 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 89323-D50 50 μg of DNA in Tris buffer $495
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DNA 89323-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pIRES2 DsRED-Express2
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 5265
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    spastin
  • Alt name
    SPAST
  • Alt name
    NM 014946
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1929
  • Mutation
    1-194 bp of 5'UTR deleted
  • Entrez Gene
    SPAST (a.k.a. ADPSP, FSP2, SPG4)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Eco47III (destroyed during cloning)
  • 3′ cloning site BglII (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer pCDH-rev
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The endogenous M1 Kozak sequence was replaced with an optimal Kozak sequence to prevent leaky translation of M87 spastin. To detect spastin use an anti-spastin antibody.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIRES RED/SP M1C448Y was a gift from Peter Baas (Addgene plasmid # 89323 ; http://n2t.net/addgene:89323 ; RRID:Addgene_89323)

  • For your References section:

    Pathogenic mutation of spastin has gain-of-function effects on microtubule dynamics. Solowska JM, D'Rozario M, Jean DC, Davidson MW, Marenda DR, Baas PW. J Neurosci. 2014 Jan 29;34(5):1856-67. doi: 10.1523/JNEUROSCI.3309-13.2014. 10.1523/JNEUROSCI.3309-13.2014 PubMed 24478365
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