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Addgene

pIRES RED/SP M87C448Y
(Plasmid #89324)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 89324 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pIRES2 DsRED-Express2
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 5265
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    spastin
  • Alt name
    SPAST
  • Alt name
    NM 014946
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1668
  • Mutation
    1-460 deleted
  • Entrez Gene
    SPAST (a.k.a. ADPSP, FSP2, SPG4)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Eco47III (destroyed during cloning)
  • 3′ cloning site BglII (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer pCDH-rev
  • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The endogenous M87 Kozak sequence was replaced with an optimal Kozak sequence. To detect spastin use an anti-spastin antibody.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIRES RED/SP M87C448Y was a gift from Peter Baas (Addgene plasmid # 89324 ; http://n2t.net/addgene:89324 ; RRID:Addgene_89324)
  • For your References section:

    Pathogenic mutation of spastin has gain-of-function effects on microtubule dynamics. Solowska JM, D'Rozario M, Jean DC, Davidson MW, Marenda DR, Baas PW. J Neurosci. 2014 Jan 29;34(5):1856-67. doi: 10.1523/JNEUROSCI.3309-13.2014. 10.1523/JNEUROSCI.3309-13.2014 PubMed 24478365