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pSUPER-retro-puro-Smad4
(Plasmid #89829)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 89829 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pSUPER-puro
  • Backbone manufacturer
    oligoengine
  • Backbone size w/o insert (bp) 4400
  • Total vector size (bp) 4400
  • Vector type
    Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Smad4 shRNA
  • gRNA/shRNA sequence
    GGTGTGCAGTTGGAATGTA
  • Species
    H. sapiens (human), M. musculus (mouse)
  • GenBank ID
    NM_005359.5
  • Entrez Gene
    SMAD4 (a.k.a. DPC4, JIP, MADH4, MYHRS)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSUPER-retro-puro-Smad4 was a gift from Shengyu Yang (Addgene plasmid # 89829 ; http://n2t.net/addgene:89829 ; RRID:Addgene_89829)
  • For your References section:

    Fascin protein is critical for transforming growth factor beta protein-induced invasion and filopodia formation in spindle-shaped tumor cells. Sun J, He H, Xiong Y, Lu S, Shen J, Cheng A, Chang WC, Hou MF, Lancaster JM, Kim M, Yang S. J Biol Chem. 2011 Nov 11;286(45):38865-75. doi: 10.1074/jbc.M111.270413. Epub 2011 Sep 13. 10.1074/jbc.M111.270413 PubMed 21914811