pLVX CUL4B 3xFlag IRES puro
(Plasmid
#90437)
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PurposeLentiviral expression of CUL4B
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 90437 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLVX IRES Puro
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCUL4B
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SpeciesH. sapiens (human)
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Entrez GeneCUL4B (a.k.a. CUL-4B, MRXHF2, MRXS15, MRXSC, SFM2)
- Promoter CMV
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Tag
/ Fusion Protein
- 3XFLAG (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLVX CUL4B 3xFlag IRES puro was a gift from Deepak Nijhawan (Addgene plasmid # 90437) -
For your References section:
Anticancer sulfonamides target splicing by inducing RBM39 degradation via recruitment to DCAF15. Han T, Goralski M, Gaskill N, Capota E, Kim J, Ting TC, Xie Y, Williams NS, Nijhawan D. Science. 2017 Mar 16. pii: eaal3755. doi: 10.1126/science.aal3755. 10.1126/science.aal3755 PubMed 28302793