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LARG mtq sspB in pcDNA3.1
(Plasmid #90460)


Item Catalog # Description Quantity Price (USD)
Plasmid 90460 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5428
  • Total vector size (bp) 7687
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    LARG DH-PH (aa 766–1138)
  • Alt name
    Rho guanine nucleotide exchange factor 12 (ARHGEF12)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • GenBank ID
  • Entrez Gene
    ARHGEF12 (a.k.a. LARG, PRO2792)

Gene/Insert 2

  • Gene/Insert name
  • Alt name
  • Insert Size (bp)
  • Mutation
    deletion a.a. (234-239)

Gene/Insert 3

  • Gene/Insert name
  • Species
  • Insert Size (bp)
  • Mutation

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The mcherry in Larg mcherry sspB was removed by cutting with BspE1 and replaced with an mTurquoise G block cut with BspE1.

LARG sequence contains an Y830F variant that does not affect plasmid function. The sspB sequence was obtained from pLL7.0: mTiam1(64-437) tgRFPt-SSPBR73Q (Plasmid #60418).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LARG mtq sspB in pcDNA3.1 was a gift from Narasimhan Gautam (Addgene plasmid # 90460 ; ; RRID:Addgene_90460)
  • For your References section:

    Membrane Flow Drives an Adhesion-Independent Amoeboid Cell Migration Mode. O'Neill PR, Castillo-Badillo JA, Meshik X, Kalyanaraman V, Melgarejo K, Gautam N. Dev Cell. 2018 Jun 15. pii: S1534-5807(18)30422-2. doi: 10.1016/j.devcel.2018.05.029. 10.1016/j.devcel.2018.05.029 PubMed 29937389