PurposeExpresses pcDNA3 - CMVmin - 5X UAS - pFR - CMVmin Luciferase
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||90492||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Gene/Insert nameCMVmin Luciferase Fluc
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
Luciferase contains a G203V mutation, which does not affect plasmid function as described in the associated publication.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pPKm-202 was a gift from Todd Coleman (Addgene plasmid # 90492 ; http://n2t.net/addgene:90492 ; RRID:Addgene_90492)
For your References section:Biosynthesis of Orthogonal Molecules Using Ferredoxin and Ferredoxin-NADP(+) Reductase Systems Enables Genetically Encoded PhyB Optogenetics. Kyriakakis P, Catanho M, Hoffner N, Thavarajah W, Hu VJ, Chao SS, Hsu A, Pham V, Naghavian L, Dozier LE, Patrick GN, Coleman TP. ACS Synth Biol. 2018 Feb 16;7(2):706-717. doi: 10.1021/acssynbio.7b00413. Epub 2018 Jan 24. 10.1021/acssynbio.7b00413 PubMed 29301067