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pNCS mOrange
(Plasmid #91764)


Item Catalog # Description Quantity Price (USD)
Plasmid 91764 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    modified pRSETB
  • Backbone manufacturer
    Life Technologies
  • Backbone size w/o insert (bp) 2900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    E. coli expressing T7 polymerase necessary for fluorescent protein production. Expresses fluorescent protein constitutively. Do not add IPTG.
  • Copy number
    High Copy


  • Gene/Insert name
    monomeric Orange
  • Alt name
  • Species
    Discosoma sp. (mushroom coral)
  • Insert Size (bp)
  • Promoter T7
  • Tag / Fusion Protein
    • Hexa-Histidine tag, Xpress epitope for detection with the anti-Xpress antibody, and enterokinase cleavage site. (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7 Promoter Forward
  • 3′ sequencing primer T7 Terminator
  • (Common Sequencing Primers)

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

These plasmids are used in University Molecular Biology Laboratories and by High School students to purify fluorescent proteins and create fluorescent protein plate artwork.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNCS mOrange was a gift from Erik Rodriguez & Roger Tsien (Addgene plasmid # 91764 ; ; RRID:Addgene_91764)
  • For your References section:

    Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Shaner NC, Campbell RE, Steinbach PA, Giepmans BN, Palmer AE, Tsien RY. Nat Biotechnol. 2004 Dec;22(12):1567-72. Epub 2004 Nov 21. 10.1038/nbt1037 PubMed 15558047