PurposeFLIM sensor for PKA activation in cytosol
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||96897||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert nameFLIM Sensor for PKA Activity in Cytosol
Insert Size (bp)2300
- Promoter CMV
/ Fusion Proteins
- sREAChet (N terminal on insert)
- EGFP (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (unknown if destroyed)
- 3′ cloning site NotI (unknown if destroyed)
- 5′ sequencing primer CMV Forward
- 3′ sequencing primer CCCTGAACCTGAAACATAAAATG (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byOur lab (EKAR), Michiyuki Matsuda (AKAR3EV)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Harvey, C.D., Ehrhardt, A.G., Cellurale, C., Zhong, H., Yasuda, R., Davis, R.J., and Svoboda, K. (2008). A genetically encoded fluorescent sensor of ERK activity. Proc. Natl. Acad. Sci. USA 105, 19264–19269.
Komatsu, N., Aoki, K., Yamada, M., Yukinaga, H., Fujita, Y., Kamioka, Y., and Matsuda, M. (2011). Development of an optimized backbone of FRET biosensors for kinases and GTPases. Mol. Biol. Cell 22, 4647–4656.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:AKARet-cyto was a gift from Ryohei Yasuda (Addgene plasmid # 96897 ; http://n2t.net/addgene:96897 ; RRID:Addgene_96897)
For your References section:Imaging ERK and PKA Activation in Single Dendritic Spines during Structural Plasticity. Tang S, Yasuda R. Neuron. 2017 Mar 22;93(6):1315-1324.e3. doi: 10.1016/j.neuron.2017.02.032. Epub 2017 Mar 9. 10.1016/j.neuron.2017.02.032 PubMed 28285819